摘要
以Fmoc-氨基酸为原料,Fmoc-Gly-Wang树脂为载体,按照氨基酸序列从C端到N端进行依次耦合得Arg34GLP-1(7-37),经由谷氨酸介导的肽链棕榈酰化,制备得利拉鲁肽。经RP-HPLC纯化得到纯度为95%以上的样品,总收率为12.8%。
Arg^34GLP-l （7-37） was synthesized successfully trom t2-termmat to N-terminal acid sequence by using Fmoc-amino acids as raw materials and Fmoc-Gly-Wang resin as a solid support. Then liraglutide was prepared by palmitoylation of the peptide chain mediated via glutamic acid. The purity of liraglutide after RP-HPLC separation was higher than 95 % and the total yield was 12.8 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2013年第2期121-124,共4页
Chinese Journal of Pharmaceuticals
关键词
利拉鲁肽
固相合成
2型糖尿病
棕榈酰化
liraglutide
solid-phase synthesis
type 2 diabetes
palmitoylation
