摘要
结合室内生测、温室盆栽和大棚内小区试验的方法,探讨了寒区昆虫病原线虫(entomopathogenic nematode,EPN)对我国北方越冬韭菜迟眼蕈蚊Bradysiaocloriphaga Yanget Zhang的防治作用。室内生测的结果显示,在侵染剂量为400条侵染期线虫(IJs)每头3龄幼虫或蛹时,异小杆线虫Heterorhabditis bacteriophora ZT(Hb.ZT,哈尔滨市植物园分离株)、H.bacteriophoraLG(Hb-LG,辽宁分离株)、H.bacteriophora NJ(Hb—NJ,美国新泽西分离株)和斯氏线虫Steinernema carpocapsaeAll(Sc—A11)对迟眼蕈蚊3龄幼虫的致死率于72h后均达100%,而对蛹的寄生效果则是Hb-ZT线虫最高;Hb—ZT与Sc—All(1:1)混用,对幼虫的室内寄生效果高于单独施用2个线虫的寄生效果;温室盆栽试验中喷施Hb.ZT与Sc—All(1:1)的线虫混合液,施用剂量减半(50万LJs/m2),对韭蛆成虫羽化的控制效果稍高于单独施用2个线虫(100万IJs/m2)效果;不同时期防治韭蛆的试验结果表明,于韭菜棚内发现韭蛆成虫5~10d内喷施线虫的防治效果较好;韭菜大棚内喷施剂量为50万LJs/m2的Hb-ZT与Sc·All线虫混合液对韭蛆幼虫防效达100%,增产作用与常规化学药剂(辛硫磷)相当。
The root maggot, Bradysia odoriphaga Yang et Zhang, is one of the most important insect pests of Chinese chives in northern China. To find alternatives to chemical pesticides for the insect, we have evaluated 3 isolates of Heterorhabditis bacteriophora, Hb-ZT (Heilongjiang), Hb-LG (Liaoning), and Hb-NJ (New Jersey, USA ), and Steinernema carpocapsae All (Sc-All) against 3rd instars and pupae of B. odoriphaga in petri dish, pot and greenhouse assay. We found that the mixed formulation of Hb-ZT and Sc-All achieved 100% , 80. 6% , and 100% host mortality in petri dish, pot, and greenhouse assay respectively, which is more effective than using each species alone. In both pot assay and greenhouse assay, the mixed formulation achieved better results with a dose of 0. 5 million IJs/m2 than each species alone at doses of 1 million IJs/m2. Our results also shown that the best time for application of entomopathogenic nematodes is 5 to 10 days after the first adults are detected.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2013年第1期235-241,共7页
Chinese Journal of Applied Entomology
基金
中国科学院知识创新工程重要方向项目(KSCX2-YW-N-092)
科技部农业科技成果转化资金项目(2007GB24910482)
黑龙江省青年科学基金项目(QC2010070)
关键词
昆虫病原线虫
韭菜迟眼蕈蚊
寒区
防效
entomopathogenic nematode, Bradysia odoriphaga, control efficacy, Chinese chive and root maggot