摘要
目的应用小动物活体成像技术观察牛磺酸（Tau）在重型颅脑创伤裸鼠体内的分布及代谢。方法将32只雄性BALB／C裸鼠随机分为正常（normal）组、假手术（sham）组、牛磺酸治疗（TBI·Tau）组和脑创伤染料组（TBI·Cy7）。后两组用液压打击仪制作左侧重型脑创伤模型，sham组在相同位置只开骨窗，不予打击。TBI-Tau、sham组伤后立即尾静脉注射Cy7．Tau，TBI。Cy7组注射游离Cy7，normal组不做处理，直接注射Cy7-Tau，剂量均为5mg／kg。给药后1、2．4、8、12、24h行活体成像。结果各组给药后体内荧光强度灰度值12h内呈线性下降，Tau主要通过肾脏代谢；sham组手术切口皮缘24h仍有较高浓度Tau富集（0．0340±0．0058）；TBI．Tau组1、2．4、8h体内荧光灰度值均强于sham组（P〈0．05），24h离体脑组织成像显示TBI-Tau组脑创伤处有高浓度Tau富集（0．640±0．096，与sham组比较，P〈0．01）。结论活体成像技术可实时监测Tau在颅脑创伤裸鼠体内的分布、代谢，Tau主要通过肾脏代谢，在脑创伤区域、皮肤切口处有特异性富集并长时间存在。
Objective To conduct an in vivo optical imaging analysis of the metabolism and biodistribution of taurine （Tan） in nude mice with severe traumatic brain injury （TBI）. Methods Nude mice were randomly divided into normal group, sham operation group, TBI group, and Tan-treated group. TBI models were made by fluid-percussion. All liquid used was injected via caudal vein immediately after TBI. In vivo optical imaging system was used to observe the distribution of metabolism of Tau at 1,2, 4, 8, 12 and 24 h after injection. Results The gray values of all groups were declined linearly after injection. The majority of Cy7-Tau was excreted through the kidney within 12 h. As compared with the normal group, Cy7-Tau in sham operation group still accumulated at skin incision margin at 24th h after injection （0. 0340 ± 0. 0058 ） ; The gray value of whole body in Tan group was stronger than in sham operation group at 1, 2, 4 and 8 h （ P 〈 0. 05 ）. Moreover,＇ high intensity Cy7-Tan at brain trauma area could last for more than 24 h （0. 64 ± 0. 096,P〈0. 01 in comparison to sham operation group）. In TBI group, Cy7 diffused subcutaneously, without concentration in any organ. Conclusion The in vivo optical imaging system can accurately monitor the distribution of metabolism of Tau. Cy7-Tau could accumulate specifically at brain traumatic area and skin incision margin.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第1期86-88,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30973089）
天津市应用基础及前沿技术研究计划资助项目（10JCYBJC12200）
关键词
颅脑创伤
牛磺酸
分布
活体成像
Traumatic brain injury
Taurine
Distribution
In vivo imaging system
