摘要
目的探讨特发性肺纤维化(IPF)患者血管紧张素转换酶(ACE)基因多态性与其血清ACE浓度的关系。方法采用聚合酶链反应(PCR)技术检测ACE基因I/D多态性,应用ELISA法测定血清ACE的浓度。结果 IPF患者的血清ACE浓度与对照组比较明显降低,差异有统计学意义(P<0.05),其基因多态性与血清ACE水平有关,ACE水平由高到低依次为:II基因型、ID基因型、DD基因型。结论 ACE基因多态性是决定IPF患者血清ACE水平的重要因素。
Objective To study the association between angiotensin converting enzyme（ACE） gene polymorphism and ACE ac- tivity in idiopathic pulmonary fibrosis patients. Methods ACE genotype of I/D was determined by polymerase chain reaction （PCR） ,and the ACE level of the serum was measured by ELISA method. Results Compared with control group, the serum ACE level of the IPF patients was decreased significantly（P〈0.05）, ACE gene polymorphism was associated with the ACE activity of the serum,the highest serum ACE activity was in genotype Ⅱ,followed by genotype ID and gene DD. Conclusion ACE gene poly- morphism is important factor to determine the ACE activity in idiopathic pulmonary fibrosis patients.
出处
《国际检验医学杂志》
CAS
2012年第21期2563-2564,共2页
International Journal of Laboratory Medicine
基金
河北省科学技术研究与发展计划资助项目(112761153)
关键词
肺纤维化
血管紧张素转换酶
基因多态性
pulmonary fibrosis
angiotensin converting enzyme
gene polymorphism
