摘要
目的探讨酸浆醇提取物(ethanol extracts of physalis alkekengi linn,EPL)能否诱导食管癌细胞株EC-1.71凋亡。方法用显微镜观察经EPL作用24、48、72 h后的EC-1.71细胞生长状况;流式细胞术检测EC-1.71细胞线粒体膜电位的变化及细胞凋亡率;western blot检测EC-1.71细胞PARP(poly ADP-ribose polymerase)裂解状况。结果在EPL作用下,EC-1.71细胞数量减少,细胞间接触疏松,凋亡细胞随着药物作用时间的延长而增多。流式细胞术结果表明,EPL作用后的细胞线粒体膜电位(△ψm)降低。与对照组比较,差异有统计学意义(P均<0.05)。作用48、72 h的细胞凋亡率随药物作用时间的延长而增高(P均<0.05)。western blot结果表明,在EPL作用48 h时可检测到相对分子量(Mr)为89 000的PARP片段。结论 EPL可以诱导EC-1.71细胞发生凋亡,其凋亡程度呈时间依赖性。
Objective To investigate whether ethanol extracts of physalis alkekengi linn （EPL） could induce the apoptosis of human e- sophageal carcinoma cell strain EC-1.71. Methods After EC-1.71 cells were incubated with EPL for 24, 48 and 72 hours respectively, their growth state was observed by microscope, the changes of mitochondria membrane potential （A＇,Itm） and the rate of apoptosis were determined by flow cytometry, and the clearage of poly ADP-ribose polymerase （PARP） were measured by Western blot. Results After EC-1.71 cells were treated with EPL, their amount was reduced, the cell-cell contact loosed, and apoptotic cells increased with the prolong of incubation time. Flow cytometry demonstrated that the A^m of EC-1.71 treated with EPL was significantly lower than that of the control group （P 〈 0.05）. Western blot indicated that the fragment of PARP with 89 000 dahons of relative molecular weight （Mr） could be detected when EC-1.71 cells were treated with EPL for 48 hours. Conclusion EPL could induce the apoptosis of EC-1.71 cells in a time-dependent manner.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2012年第12期983-986,共4页
Chinese Journal of Clinical Laboratory Science
关键词
酸浆醇取物
食管癌
细胞凋亡
PARP
线粒体膜电位
ethanol extracts of physalis alkekengi linn
esophageal carcinoma
apoptosis
poly ADP-ribose polymerase
mitochondria membrane potential
