摘要
试验选取平均体质量为536.67(±15.23)g的褐牙鲆,随机分为6个平行组,饥饿处理0、2、10、20 d,研究饥饿胁迫对褐牙鲆血浆皮质醇、溶菌酶(LSZ)活性及免疫组织中非特异免疫酶活性的影响。结果显示:饥饿0～20 d过程中,随着饥饿时间的延长,血浆皮质醇浓度呈现增加趋势,并且在饥饿10、20 d时与饥饿前相比有显著性差异,而肝脏与脾脏酸性磷酸酶(ACP)活性呈现下降的趋势,并且在饥饿10、20 d时与饥饿前相比存在显著性差异。血浆LSZ活性、肝脏超氧化物歧化酶(SOD)活性、肝脏与脾脏碱性磷酸酶(AKP)活性随着饥饿时间的延长,均呈现先上升后下降的趋势,在饥饿2 d时与饥饿前相比显著升高,在饥饿20 d时均降低,并且肝脏SOD与AKP活性与饥饿前相比均有显著性差异。饥饿0～20 d过程中,脾脏与肾脏LSZ活性、肾脏AKP活性、肾脏ACP活性与饥饿前相比没有显著性差异。结果表明,短期(2 d)饥饿胁迫可激活褐牙鲆非特异免疫酶的活性,而长期(20 d)饥饿胁迫能够降低褐牙鲆免疫机能,同时皮质醇激素可能对褐牙鲆非特异免疫系统具有调控作用。
In order to estimate effects of starvation on cortisol concentration and non-specific immunity of Japanese flounder （Paralichthys olivaceus）, Japanese flounder with body weight（536,67±15.23）g were randomly divided into six groups, then were starved for 0 d, 2 d, 10 d, 20 d. The results showed that with the increasing of starvation, the plasma cortisol concentrations appeared increasing and presented significant difference in the starvation for 10 d and 20 d, while the liver and spleen acid phosphatase （ACP） activities became decreasing and were significant difference in the starvation for 10 d and 20 d. The plasma lysozyme （LSZ）activities, liver superoxide dismutase （SOD） activities, liver and spleen alkaline phosphatase （AKP） activities appeared the trend of increasing, then decreasing of which were significant difference in the starvation for 2 d, and of which were difference in the starvation for 20 d, especially the liver SOD and AKP activities were significant difference. There was no significant difference in the spleen and kidney LSZ activities, kidney AKP activities and kidney ACP activities. The results indicated that short term （2 d） starvation could activate the non-specific immune enzyme activities of Japanese flounder, while long term （20 d） starvation could decease the immunity of fish, maybe the cortisol regulate the non- specific immune system.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第24期134-137,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31072228)
上海市科委项目(11PJ1404500)
关键词
褐牙鲆
饥饿
皮质醇
非特异免疫
Paralichthys olivaceus
starvation
cortisol
non-specific immunity
