摘要
以20份源于欧洲和亚洲的芜菁(Brassca campestris L.ssp.rapifera Matzg)为研究试材,采用高效液相色谱法对日光温室条件下生长的芜菁叶片中硫代葡萄糖苷(硫苷)的组分和含量进行了测定。每份材料中均检测到8种硫苷成分,其中3种为脂肪族硫苷,4种为吲哚族硫苷,1种为芳香族硫苷。脂肪族硫苷含量最高,占总硫苷含量的85.26%,并且总硫苷和单一硫苷在不同材料间存在差异较大。相关性分析结果显示,NAP与GBN、NAP和GBN与总硫苷间均呈极显著正相关。主成分分析表明脂肪族硫苷NAP、GBN和PRO是芜菁中硫苷的主要组分,同时筛选出4份高硫苷含量的芜菁材料。
Twenty Brassica campestris L.ssp.rapifera Matzg materials were used to identify glucosinolate profile and accumulation in leaves by high performance liquid chromatography（HPLC） analysis.Eight glucosinolates were detected in all the accessions,three of which were aliphatic glucosinolates,four of which were indolic glucosinolates and one of which were aromatic glucosinolate.The aliphatic glucosinolates were dominant in turnip with the percentage of 85.26%.The total glucosinolates and individual glucosinolate contents varied within varieties.Correlation analyses showed that the accumulation of different glucosinolates was affected by each other,and correlations between NAP and total glucosinolate content,GBN and total glucosinolate,between NAP and GBN were significant.Principal component analysis indicated that aliphatic glucosinolates（NAP,GBN and PRO） dominated glucosinolate profiles,and 4 materials with high contents of glucosinolate were identified.
出处
《江苏农业学报》
CSCD
北大核心
2012年第6期1413-1417,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家"973"计划项目(2007CB108803)
江苏省农业科技自主创新基金项目[CX(11)3046和CX(11)1006]
关键词
芜菁
硫代葡萄糖苷
构成
含量
高效液相色谱法
Brassica campestris L.ssp.rapifera Matzg
composition
content
glucosinolate
high performance liquid chromatography
