摘要
采用高效液相色谱法,以64份普通白菜DH系为试材,对不同季节条件下生长的普通白菜叶片中硫代葡糖糖苷(简称硫苷)的组分和含量进行了研究。在64份普通白菜DH系中均检测到8种硫苷组分(3种脂肪族硫苷、4种吲哚族硫苷和1种芳香族硫苷),脂肪族硫苷含量最高,占总硫苷含量的80%以上,其中NAP含量最高,达50%,同时发现1份硫苷含量极低的材料。不同材料间脂肪族硫苷变异幅度最大,变异系数均在100%以上。相关性分析发现,不同季节间脂肪族硫苷、芳香族硫苷和总硫苷含量均达显著或极显著正相关。方差分析表明,脂肪族硫苷、芳香族硫苷和总硫苷含量两个季节中不同基因型间差异均达极显著水平。基因型与季节之间的联合方差分析显示,吲哚族硫苷在基因型间均不存在显著差异,但多种硫苷存在明显的季节×基因型交互作用。
The glucosinolate profile and accumulation of 64 Brassca campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee DH lines grown in different seasons were analyzed with high performance liquid chromatography(HPLC).Eight main glucosinolates(3 aliphatics,4 indoles and 1 aromatic)were detected in all accessions.The aliphatic glucosinolates were the most dominant glucosinolates in pack choi with the highest ratio of above 80 %,and gluconapin(NAP)is about 50 %,in addition,1 accession with low glucosinolate was detected.Coefficient variability index in each aliphatic glucosinolate was more than 100 %.Correlations analysis between individual glucosinolates showed that the correlation of aliphatic,aromatic and total glucosinolate was all significant or very significant respectively between the 2 seasons.Variance analysis on glucosinolate accumulation showed that there were very significant differences among genotypes in aliphatic,aromatic and total glucosinolate among genotypes in the 2 seasons.Combined variance analysis indicated that there were no significant differences among genotypes in indole glucosinolates,and season×variety interaction was identified in many glucosinolate.
出处
《中国蔬菜》
北大核心
2011年第10期35-40,共6页
China Vegetables
基金
国家"973"计划项目(2007CB108803)
农业部园艺作物遗传改良重点开放实验室项目
关键词
普通白菜
硫代葡萄糖苷
高效液相色谱法
Brassca campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee
Glucosinolate
HPLC
