摘要
以苏丹草2098与拟高粱杂交种F1(对照)及秋水仙碱处理的苏丹草与拟高粱杂交种胚性愈伤组织获得的再生植株SS2010-1为材料,通过苗期植株形态学特征观察,结合根尖细胞染色体计数和流式细胞仪对再生植株SS2010-1进行倍性鉴定,并对其叶片表皮气孔器特性进行分析。结果显示:与对照相比,SS2010-1株高和叶长分别增加63.55%和56.12%(P<0.05),叶片宽度增加6.76%(P>0.05);SS2010-1细胞核DNA含量是对照的2倍,另外,根尖细胞染色体数目为2n=40,为对照染色体数目(2n=20)的2倍,表明秋水仙素诱导获得的再生植株SS2010-1为加倍的四倍体新材料。与对照相比,SS2010-1叶片上表皮1 mm2气孔器数目减少64.39%(P<0.05),气孔器长度和宽度分别增加29.33%和15.99%(P<0.05),保卫细胞宽度增加18.66%(P<0.05);叶片下表皮气孔器密度减少39.09%(P<0.05),气孔器长度和宽度分别增加23.24%和26.19%(P<0.05),保卫细胞宽度增加3.74%(P>0.05)。
Interspecific hybrid（CK） of Sorghum sudanense（Piper）Stapf.× Sorghum propinquum（Kunth）Hitchc.and SS2010-1 regenerated from F1 embryonic callus treated by colchicine were used as experimental materials.Ploidy determination of SS2010-1 was conducted by seedling morphological characteristics analysis,chromosome number counting,and flow cytometry analysis,and the leaf stomatal apparatus characteristics of the materials were studied.Compared to those of CK,the plant height and leaf length of SS2010-1 were increased by 63.55%（P0.05）and 56.12%（P0.05）,respectively,and the leaf width of SS2010-1 was 6.76% wider（P0.05）.The nuclear DNA contents in SS2010-1 were twice as much as that of CK,and the chromosome number of SS2010-1（2n=40） was two times as much as that of CK（2n=20）.The results above indicated that the new material SS2010-1 was a tetraploidy.Compared to those of CK,the stomatal apparatus density in upper epidermis of SS2010-1 was decreased by 64.39%（P0.05）,the stomatal apparatus length and width were increased by 29.33%（P0.05） and 15.99%（P0.05）,respectively,and the guard cell width was increased by 18.66%（P0.05）.The stomatal apparatus density in lower epidermis of SS2010-1 was decreased by 39.09%（P0.05）,the stomatal apparatus length and width were increased by 23.24%（P0.05） and 26.19%（P0.05）,respectively,and the guard cell width was increased by 3.74%（P0.05）.
出处
《江苏农业学报》
CSCD
北大核心
2012年第6期1386-1391,共6页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技支撑计划项目(BE2010308)
关键词
苏丹草
四倍体
染色体
流式细胞术
气孔器特性
Sorghum sudanense（Piper）Stapf.
tetraploidy
chromosome
flow cytometry
stomatal apparatus characteristic
