摘要
以甘菊种子为试验材料,研究了不同秋水仙素浓度和处理时间对甘菊多倍体诱导的影响。结果表明:500mg/L的秋水仙素水溶液浸渍种子12h,变异率为23%,达到了极显著差异。根尖染色体压片检查表明,四倍体染色体数为2n=4x=36,并且获得了同源四倍体甘菊1株(2n=4x=36)。四倍体植株表现出叶片下表皮气孔保卫细胞变大,叶绿体数变多,叶片变小,花径变大,舌状花数目减少,管状花数目增多。
The effects of colchicines concentration and treatment time duration on the seeds of Dendranthema lavandulifolium were studied. The results showed that method for polyploidy induction was soaking seeds in 500mg /L colchicum for about 12h and the induction ratio was about 23% ,which showed significant difference. Only one tetraploidy plant was obtained with the chromosome number of 2n = 4x = 36. The tetraploid plant exhibited some morphological and cytological variations,including the larger guard cells size of stomata,more chloroplasts in stomata guard cell,smaller leaves,bigger flower diameter,less number of ray flower and more number of tubular flower.
出处
《核农学报》
CAS
CSCD
北大核心
2010年第3期527-531,共5页
Journal of Nuclear Agricultural Sciences
基金
"十一五"科技支撑项目-主要商品花卉新品种选育(2006BAD01A18)
北京林业大学新进教师科研启动基金项目-地被菊种质创新及综合评价体系建立
北京市教育委员会学科建设与研究生培养项目-北京城市生态环境建设产学研联合培养研究生基地资助(二期资助)
关键词
甘菊
秋水仙素
多倍体育种
Dendranthema lavandulifolium
colchicine
polyploidy breeding
