摘要
目的 观察B7 1基因表达对肝癌细胞增殖特性的影响及诱发杀伤性T淋巴细胞 (CTL)细胞毒活性的变化。方法 用细胞计数法和流式细胞仪 (FCM)测定细胞生长曲线、DNA含量及细胞周期的变化。同时测定CTL对转染B7 1基因前后肝癌细胞的细胞毒作用。结果 转染B7 1基因后 ,肝癌细胞的增殖受到一定影响 :生长延缓、增殖幅度降低和倍增时间延长。CTL对转染B7 1基因的肝癌细胞杀伤明显增强。结论 转染B7 1基因的肝癌细胞出现了一定的增殖抑制现象 ,提示B7
Aim To observe the effects of B71 gene expression on proliferation of hepatocarcinoma cells and on changes in cytotoxic activity of induced CTL. Methods Growth curve, DNA content and change in cell cycle were determinated by Cytometry and FCM. The cytotoxicity alteration of the CTL was also studied. Results After transfected wth B71 gene, the cell proliferation was lagged,and the cytotoxicity was reinforced. Conclusion The proliferation of HHCC after transfected with B7.1 gene can be inhibited which suggests a relationship between B71 gene and the immune system.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
2000年第3期268-270,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助!No.39770827
