摘要
目的 提高心肌保护效果 ,延长供心的保存时限 .方法 将 2 4只大白兔分为两组 ,每组 12只 ,在 St Thomas 液中加入无机质游离牛血红蛋白 ( SFBHB)作为载氧体 ,常温灌注离体兔心 6 h作为实验组 ,用单纯 St Thomas 液低温保存离体兔心作为对照组 .结果 左室发展压 [L VDP,( 13.77±1.36 ) k Pa vs( 7.2 2± 1.13) k Pa,P<0 .0 1],冠状动脉流量恢复率 [CFR,( 92 .8± 5 .16 ) % vs( 5 9.0 7± 9.5 9) % ,P<0 .0 1]均显著高于对照组 ;心肌组织 ATP含量在保存 4h后两组即有明显差异 [( 30 .1± 2 .6 ) μmol.g- 1 vs( 2 7.2± 3.7) μmol.g- 1 ,P<0 .0 5 ],复跳 30 min后 ,实验组较对照组心肌组织的ATP含量明显升高 [( 2 9.9± 3.6 ) μmol.g- 1 vs( 10 .9± 4.4)μmol.g- 1 ,P<0 .0 1];同时 ,试验组心肌氧摄取量明显高于对照组 [( 5 37.3± 12 4.0 ) nmol.s- 1 .g- 1 vs( 90 .5± 39.5 ) nmol.s- 1 .g- 1 ,P<0 .0 1],心肌组织内的丙二醛 [MDA,( 6 .2 4± 2 .10 ) μmol.g- 1 vs( 18.6 0± 5 .6 3) μm ol.g- 1 ,P<0 .0 1]含量及冠状静脉引流液中肌酸磷酸激酶 [CPK,( 1.1± 0 .1) nkat.s- 1.g- 1 vs( 3.6± 0 .5 ) nkat.s- 1 .g- 1 ,P<0 .0 1],乳酸脱氢酶[L DH,( 2 30 .4± 6 0 .9) nkat.15 min- 1 .g- 1
AIM To evaluate the effects of stroma-free bovine hemoglobin (SFBHB) on prolonging the preservation time of the donor heart. METHODS There were two groups of isolated perfused rabbit hearts. In groupⅠ (n=12, control group), the cardiac arrest was induced with St ThomasⅡ solution and repeated every 30 minutes, and the myocardial temperature remained at 4℃. In group Ⅱ (n=12, experimental group), the isolated rabbit hearts were perfused continuously with St ThomasⅡsolution containing stromafree bovine hemoglobin and the myocardial temperature remained at 37℃. The preservation period was 6 hours. RESULTS Significant differences were observed between the two groups of isolated rabbit hearts: The mean values of the left ventricular developed pressure [LVDP, (13.77±1.36) kPa vs (7.22±1.13) kPa, P<0.01] and coronary flow rate [CFR, (92.8±5.16)% vs (59.07±9.59)%, P<0.01] were higher in group Ⅱ (P<0.01); the ATP level [(10.9±4.4) μmol·g -1 vs (29.9±3.6) μmol·g -1 , P<0.01] and myocardial oxygen uptake [(537.3±124.0) nmol·s -1 ·g -1 vs (90.5±39.5) nmol·s -1 ·g -1 , P<0.01] significantly decreased in groupⅠ; the mean values of released creatine phosphate kinase [CPK, (1.1±0.1) nkat·s -1 ·g -1 vs (3.6±0.5) nkat·s -1 ·g -1 , P<0.01)], lactec dehydrogenase [LDH, (230.4±60.9) nkat·15 min -1 ·g -1 vs (533.9± 72.2 ) nkat·15 min -1 ·g -1 , P<0.01] and myocardial contents of malondialdehyde [MDA, (6.24±2.10) μmol·g -1 vs (18.60±5.63) μmol·g -1 , P<0.01] were greater in groupⅠ. The myocardial ultra structural injuries were greater in groupⅠ. CONCLUSION The stromafree bovine hemoglobin could offer greater protective effects on preservation of the isolated rabbit hearts.
出处
《第四军医大学学报》
2000年第5期576-578,共3页
Journal of the Fourth Military Medical University
