摘要
评价冷血心肌麻痹液（ＣＢＣ）温度对肌浆网（ＳＲ）Ｃａ２＋摄取和释放的影响。方法测定ＣＢＣ不同温度停搏１２０分钟和再灌注后心肌匀浆ＳＲ４５Ｃａ２＋摄取及钉红阻滞ＳＲＣａ２＋释放通道后ＳＲ４５Ｃａ２＋摄取的变化。结果停搏期１６℃和２０℃ＳＲ ４５Ｃａ２＋摄取降幅分别为１７．０９％和２１．１６％（Ｐ＜０．０５）；停搏期４、８、１２℃、再灌注后各组ＳＲ ４５Ｃａ２＋摄取与对照组差异均无显著意义（Ｐ＞０．０５）。ＳＲＣａ２＋释放通道阻滞后各组停搏和再灌注后ＳＲ摄４５Ｃａ２＋增幅差异无显著意义（Ｐ＞０．０５）。结论ＣＢＣ温度不同所表现的保护效果差异与ＳＲＣａ２＋摄入受损有关，ＳＲＣａ２＋释放不受影响。
Objective To investigate the temperature effect of cold blood cardioplegia (CBC) on cardiac sarcoplasmic reticulum (SR) Ca2+ uptake and release. Method SR Ca2+ uptake was assayed in homogenates from isolated rat heart in presence or absence of Ca2+ -release channel blockade (rethnium red,RR), in which hearts underwent cold blood cardioplegic arrest for 120 min with myocardial temperature beingmaintained at 4℃, 8℃, 12℃, 16℃ and 20℃ respectively, and followed by 45 min of reper fusion, ResultDuring arrest, SR Ca2+ uptake was markedly decreased in groups of 16t and 20t (17.09% and 21. 16%,vs control, P<0. 05), but remained in control level in groups of 4℃, 8℃ and 12℃. The percent incrementof net Ca2+ uptake produced by RR was similar in all groups. Following reperfusion, the decreased SR Ca2+.uptake during arrest in groups of 16℃ and 20℃ was restored to that of control, neither decrease of SR Ca2+uptake nor abnormal changes in percent increment of net Ca2+ uptake were found in all groups. ConclusionDuring cold blood cardioplegic arrest with various temperatures being maintained, abnormal SR Ca2+ ATPaseactivity caused by temperature may be responsible for the decreased SR Ca2+ uptake. while SR Ca2+ releasestill remains unchanged.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第2期105-107,共3页
Chinese Journal of Experimental Surgery
关键词
心肌麻痹液
温度
肌浆网
心肌保护
Cardioplegia, Temperature ,Sarcoplasmic reticulum ,Myocardial protection
