摘要
目的:对自发性高血压大鼠(SHR)行星状神经节阻滞(SGB),观察星状神经节阻滞对其左心室重构的影响。方法:将32只10周龄雄性SHR随机分为4组(每组8只):左侧星状神经节阻滞组(LS组)、右侧星状神经节阻滞组(RS组)、药物卡托普利组(D组)、手术对照组(C组)。用ALC-NIBP无创血压测量系统测定大鼠血压,第10周实验结束后用3%戊巴比妥钠腹腔注射(45 mg/kg)麻醉SHR,迅速取出心脏测左心室质量指数。HE染色后光镜下观察左心室心肌组织结构,放射免疫法测定其eNOS和ET-1的浓度,免疫组织化学法检测其I型、III型胶原蛋白表达的含量。结果:与C组和LS组比较,RS组可降低左心室质量指数(P<0.05),改善心肌组织结构,降低心肌组织中ET-1和增加eNOS的含量(P<0.05),降低I型胶原和增加III型胶原表达(P<0.05)。结论:右侧星状神经节阻滞在治疗自发性高血压大鼠的同时可改善和逆转左心室重构。
Objective： To determine the effect ofstellate in spontaneously hypertensive rats （SHRs） ganglion block on reconstruction of the left ventricle
Methods： Thirty-two lO-week-old male SHRs were randomly assigned into 4 groups： a left stellate ganglion block group （group LS）, a right stellate ganglion block group （group RS）, a captopril group （group D） and a control group （group C）. The arterial systolic blood pressure （SBP） was measured by ALC-NIBP measuring system. After 10 weeks, we observed the left ventricular mass index （LVMI）, myocardial pathologic changes, and detected the endothelin （ET-1） and endothelial nitric oxide synthase （eNOS） level in the left ventricle by radioimmunoassay and the collagen protein level in the left ventricle by immunohistochemical method.
Results： Compared with group LS and group C, the LVMI in group RS was lowered most notably （P〈0.05） and pathological changes were improved obviously. The expression of eNOS in group RS was significantly increased and ET-1 significantly decreased （P〈0.05） compared with that in group C and group LS. The expression of type I collagen fibers in group RS was significantly lower and type III collagen fibers significantly higher （P〈0.05） when compared with that in group C and LS. Conclusion： Right stellate ganglion block can not only decrease the arterial pressure but also reverse the reconstruction of the left ventricle.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期43-47,共5页
Journal of Central South University ：Medical Science
基金
安徽省教育厅自然科学研究项目(KJ2009B041Z)~~
关键词
神经阻滞
星状神经节
大鼠
自发性高血压
左心室重构
nerve block
stellate ganglion
rat, spontaneously hypertension
reconstruction of left ventricle
