摘要
目的:建立同时测定麻杏二陈汤煮散剂中盐酸麻黄碱和苦杏仁苷含量的方法。方法:采用HPLC法,色谱柱为Dia-monsil C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液(10∶10∶80),柱温为室温,检测波长为207 nm。结果:盐酸麻黄碱在0.8-40.0μg.mL-1的范围内有良好的线性关系,r=0.9999,平均加样回收率为100.53%,RSD%为1.20%(n=9);苦杏仁苷在4.04-202.00μg.mL-1的范围内有良好的线性关系,r=0.9999,平均加样回收率为100.34%,RSD%为1.42%(n=9)。结论:本法可同时测定盐酸麻黄碱和苦杏仁苷的含量,测定方法简便,准确,重复性好,可用于麻杏二陈汤煮散剂的质量控制。
Objective： To establish a method for determining the contents of ephedrine hydrochloride and amygdalin in Maxingerehen decoction made from powder. Methods： HPLC was performed to detect the contents with Diamonsil C18 col- umn（4.6 mm×250mm, 5μm）; the mobile phase was methanol-acetonitrile-0.1%phosphoric acid solution （10：10：80）; the column temperature was room temperature; the UV detection wavelength was 207 nm. Results： The liner ranges of ephedrine hydrochlo- ride and amygdalin were 0.8-40.0μg·mL-1, r=0.9999, 4.04-202.00 μg·mL-1, r--0.9999. The average recovery of ephedrine hy- drochloride was 100.53% （n=9） and RSD% was 1.20%; the average recovery of amygdalin was 100.34% （n=9） and RSD% was 1.42%. Conclusion： This method is simple, accurate and with good reproducibility. It can be used for quality control of Maxingerchen decoction made from powder.
出处
《中医药导报》
2013年第1期80-82,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广西壮族自治区卫生厅科研课题(Z2012162)
