摘要
目的:探讨电针疗法减轻缺血脑组织病理损伤的可能作用机理。方法:改良线栓法制备局灶性脑缺血再灌注模型,电针"百会"、"水沟"、"足三里",通过逆转录聚合酶链反应(RT-PCR)法半定量测定各组Survivin mRNA的表达。结果:电针组病理改变较相同时相模型组病理改变较轻。相同时相电针组Survivin mRNA的表达比模型组均要高,差异均有统计学意义(P<0.05)。结论:电针疗法可有效减轻缺血脑组织的病理损伤,其作用机理可能与增加缺血脑组织Survivin mRNA的表达有关。
Objective： To explore possible mechanism of electro-acupuncture therapy on reducing ischemic brain injury. Methods： Modified Suture-occluded method was applied for forming focal cerebral ischemia reperfusion model; the acupuncture points of Electro-acupuncture were ＂Baihui＂, ＂Shuigou＂ and ＂Zusanli＂; reverse transcriptase polymerase chain reaction （RT- PCR） method was used for semi quantitative determination of serum Survivin mRNA expression. Results： The pathological change in the treatment group was lighter than that model group in the same phase; in the same phase, the Survivin mRNA expression of acupuncture group were higher than that of the model group, with statistically significant differences （P〈0.05）. Conclusion： Electro-acupuncture therapy can provide effect of reducing ischemic brain injury, the mechanism of which may be associated with the increase of Survivin mRNA expression in ischemic brain tissue.
出处
《中医药导报》
2013年第1期12-13,16,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
教育部高等学校博士学科点专项科研基金(20094230110002)
