摘要
目的调查亚胺培南耐药的铜绿假单胞菌携带整合子情况,了解其与多重耐药的相关性。方法采用Mi-cro Wlkway-96全自动细菌鉴定仪进行细菌鉴定和药敏试验,PCR法对临床分离的120株亚胺培南耐药和80株亚胺培南敏感的铜绿假单胞菌进行整合子检测。结果 120株亚胺培南耐药的铜绿假单胞菌中Ⅰ类整合子的检出率为81.7%(98/120)、Ⅲ类整合子检出率为1.7%(2/120),未检出Ⅱ类整合子;80株亚胺培南敏感的铜绿假单胞菌中Ⅰ类整合子的检出率为21.3%(17/80),未检出Ⅱ、Ⅲ类整合子。整合子阳性的铜绿假单胞菌对临床常用抗菌药物的耐药率明显高于整合子阴性的铜绿假单胞菌,并且前者多重耐药现象较为严重。结论Ⅰ类整合子广泛存在于亚胺培南耐药的铜绿假单胞菌中,并与其多重耐药性密切相关。
Objective to investigate the distribution of imipenem-resistant clinical isolates of Pseudomonas aerugino- sa. Methods Bacterial identification and susceptibility were tested by Micro-Walkway-96 automatic bacterial instru- ment. The integrons,in 120 imipenem-resistant and 80 imipenem-sensitive of Pseudomonas were aeruginosa, detected,yjby PCR. Results Ciass I and class Ⅲ integron were 81. 7% （98/120）, 1. 7% （2/120） in imipenem-resistant Pseudonas aeruginosa,respectively, class Ⅱ integron were not detected. And class I integron rate was 21.30/00 （17/80） in 80 strains of imipenem- sensitive of Pseudomonas aeruginosa ,but class Ⅱ and class Ⅲ integron were not detected. ？ Integron positive of Pseudomonas aeruginosa clinical antibiotic resistance significantly higher than integron negative of Pseudomonas aeruginosa,and the former multi- drug resistance is more serious. Conclusion Our results revealed that Class I integron were widespread in imipenem-resistant clinical isolates of Pseudomonas aeruginosa.
出处
《中国实验诊断学》
2013年第1期31-33,共3页
Chinese Journal of Laboratory Diagnosis
