摘要
目的研究医院感染铜绿假单胞菌产超广谱β-内酰胺酶(ESBLs)的基因型分布。方法用聚合酶链反应(PCR)对68株医院感染铜绿假单胞菌的SHV、TEM、CTX-M、OXA-10、VEB和PER等基因进行扩增和序列测定。结果68株医院感染铜绿假单胞菌中,检出26株产OXA-10群ESBLs,检出率为38.23%(26/68);其中1株既产OXA-10群ESBLs又产TEM型广谱β-内酰胺酶;其余基因型未检出。经序列测定为OXA-10-like基因和TEM-1基因。结论医院感染铜绿假单胞菌所产的ESBLs主要为OXA-10群,且为多重耐药菌株。
Objective To study the genotype of extended spectrum beta-lactamases （ESBLs） among Pseudomonas aeruginosa in nosocomial infection. Methods SHV, TEM, CTX-M, OXA-IO, VEB and PER gene of 68 nosocomial isolates of Pseudomonas aeruginosa were detected by polymerase chain reaction （PCR）. Some of the products were sequenced. Results Among nosocomially isolated 68 Pseudornonas aeruginosa, 26 strains had the OXA-IO groups ESBLs geue with a positive rate of 38.23 % （26/68）, and one strain had both OXA-10 groups ESBLs and TEM broad-spectrum beta-lactamase gene. Other genes were all negative. OXA-10-1ike and TEM-1 gene were found by sequencing some PCR amplified products. Conclusions Pseudomonas aeruginosa in nosocomial infection mainly produce OXA-IO groups ESBLs, and all the strains are multiple resistant.
出处
《检验医学》
CAS
北大核心
2009年第1期9-12,共4页
Laboratory Medicine
基金
温州医学院重大科研课题B类项目基金资助(EXYZD-B2005-08)
关键词
铜绿假单胞菌
超广谱Β-内酰胺酶
基因型
医院感染
Pseudomonas aeruginosa
Extended spectrum beta-lactamase
Genotype
Nosocomial infection
