摘要
目的研究在增殖性糖尿病视网膜病变患者玻璃体与血清中SDF-1和VEGF的浓度及其相互关系。方法本研究选取22例因增殖性糖尿病视网膜病变而行玻璃体切割手术患者的玻璃体及血清为实验组,同时取13例角膜移植的供体眼的玻璃体和13例体检健康者血清标本为对照组。实验组22例患者中2年内有否接受过全视网膜光凝分为PRP组和无激光治疗组。采用ELISA法定量测定各组玻璃体与血清中SDF-1、VEGF的表达水平,并进行统计学分析。结果 PDR组患者玻璃体SDF-1、VEGF浓度分别为179.07±80.64、1721.19±1021.59pg/ml,明显高于对照组62.00±19.84、98.80±37.88pg/ml(P<0.001)。正常对照组、无激光治疗组及PRP组3组间SDF-1、VEGF浓度差异具有显著性(F=21.61,F=16.39,P<0.001)。其中,PRP组的PDR患者玻璃体SDF-1、VEGF含量均低于无激光治疗组的PDR患者,差异具有统计学意义(P<0.005)。22例PDR患者玻璃体内VEGF与SDF-1、VEGF的浓度呈正相关,玻璃体与血清内SDF-1、VEGF浓度呈正相关。结论 SDF-1与VEGF参与了PDR患者病理性视网膜新生血管的形成过程。全视网膜光凝可有效降低SDF-1、VEGF浓度,抑制网膜新生血管生长。
Objective To investigate the relevance of SDF - 1 and VEGF in the vitreous and serum in the patients with proliferative diabetic retinopathy. Methods We used the vitreous and serum from twenty - seven patients who had proliferative diabetic retinopathy and vitrectomy as the research group. The vitreous from ocular donor during the corneal transplant and the serum from the healthy person who took part in the healthy examination were in the control group. The volumes of SDF - 1 and VEGF in the vitreous and serum were measured by enzyme -linked immunosorbent assay. We also divided 22 patients in research group into two subgroups： PRP group and Non - PRP group. Results The vitreous concentration of SDF - 1 and VEGF in eyes with PDR were 179.07 ± 80.64 and 1721.19 ± 1021.59pg/ml,respectively, which were significantly higher than that in control group 62.00 ± 19.84 and 98.80 ± 37.88 pg/ml（ P 〈 0. 001 ） ,respectively. The difference of SDF - 1 and VEGF among the three groups （ Non - PRP group, PRP group and control group） was significant（ F =21.61 ,F = 16.59, P 〈 0. 001 ）. The levels of SDF - 1 and VEGF in PRP group were much lower than the Non - PRP group（P 〈 0. 005）. The vitreous concentration of SDF- 1 correlated significantly with that of VEGF in eyes with PDR. The vitreous concentration of SDF - 1, VEGF correlated significantly with the serum level of SDF - 1 in patients with PDR. Conclusion The vitreous levels of SDF - 1 and VEGF were increased in eyes with PDR. SDF - 1 and VEGF may be correlated with angiogenesis of PDR. PRP may decrease vitreous concentration of SDF - 1 and VEGF, and inhibit angiogenesis of PDR.
出处
《医学研究杂志》
2013年第1期100-104,共5页
Journal of Medical Research
基金
温州市科技局对外科技合作交流项目(H20100012)
关键词
糖尿病视网膜病变
基质细胞衍生因子
血管内皮生长因子
Diabetic retinopathy
Stromal cell - derived factor - 1 （ SDF - 1 ）
Vascular endothelial growth factor （VEGF）
