摘要
目的研究食管鳞癌(ESCC)、食管-胃交界部癌(CEGJ)、胃癌(GC)组织中人表皮生长因子受体2(HER2)基因扩增情况及其临床病理意义。方法随机选取ESCC、CEGJ、GC根治术后的切除标本76、50、48例和相应癌旁正常上皮组织各21例,应用荧光原位杂交(FISH)技术检测HER2基因扩增水平。结果 HER2基因扩增率在ESCC、CEGJ、GC分别为3.9%(3/76)、24.0%(12/50)、18.8%(9/48),在正常上皮组织均不扩增。ESCC的HER2基因扩增与肿块局部浸润、脉管累犯、淋巴结转移正相关(P<0.05),CEGJ、GC基因扩增与患者性别、年龄、分化程度、局部浸润、脉管累犯、淋巴结转移无关(P>0.05)。与ESCC比较,CEGJ与GC的生物学行为更相似。结论HER2基因在CEGJ和GC中的扩增可能和肿瘤发生有关,有可能成为靶向治疗的靶点。ESCC的HER2基因扩增率较低,但如有扩增,提示肿瘤更易转移。
Objective To investigate the amplification of human epidermal growth factor receptor-2（HER2） gene in esophageal squamous cell carcinoma（ESCC）, cancer of esophago-gastric junction（CEGJ） and gastric carcinoma （GC） and their relationships with clinicopathological parameters. Methods Amplification of HELL2 gene was examined by FISH in 76 specimens of ESCC, 50 specimens of CEGJ, 48 specimens of GC. The 21 specimens of tumor-adjacent normal epithelium from each cancer cases above were taken as the controls. Results The amplification rates of HER2 gene in ESCC,CEGJ and GC were 3. 9%（3/76） ,24. 0%（12/50） and 18. 8%（9/48） ,and there was no gene amplification in tumor-adjacent normal epithelium. In ESCC, amplification of HER2 gene was positively correlated with invasion of ESCC cells, vascular recidivism and lymph node metastasis （P〈0. 05）. However,amplification of HER2 gene in CEGJ and GC was not correlated with gender, age,differentiation degree, cell invasion, vascular recidivism and lymph node metastasis （P〉0. 05）. Compared with ESCC, the biological behavior of CEGJ was similar to that of GC. Conclusion The amplification of HER-2 gene may be related to tumorigenesis of CEGJ and GC and could be a new target for targeted therapy. The amplification rate of HER-2 gene in ESCC is low, but will promote tumor metastasis if HER-2 is amplified.
出处
《江苏医药》
CAS
北大核心
2013年第1期27-29,共3页
Jiangsu Medical Journal
基金
江苏省"333"工程科研资助项目[苏人才办(2009)24号]
关键词
食管鳞癌
食管-胃交界部癌
胃癌
人表皮生长因子受体2
Esophageal squamous cell carcinoma
Cancer of esophago-gastric junction
Gastric carcinoma
Human epidermal growth factor receptor-2
