摘要
目的:探讨食管鳞癌中EGFR信号传导通路相关分子EGFR、HER2的表达情况及二者之间的相互关系,对比研究免疫组织化学方法(IHC)检测食管鳞癌HER2蛋白表达和荧光原位杂交技术(FISH)检测HER2基因状态的一致性。方法:随机选取食管鳞癌根治术后的切除标本76例和21例癌旁正常鳞状上皮组织,应用IHC法检测EGFR的表达。IHC法及FISH法分别检测HER2蛋白表达和HER2基因状态,并进行对比分析。结果:EGFR在食管鳞癌的表达明显高于癌旁正常鳞状上皮(63.2%vs 33.3%,P<0.05);HER2在食管鳞癌低表达(3.9%),正常鳞状上皮不表达。EGFR、HER2表达均与脉管累犯、淋巴结转移正相关,此外,HER2表达还与肿瘤浸润深度正相关。HER2与EGFR表达未发现相关性;HER2蛋白为阳性的患者中,HER2基因扩增符合率为66.7%(2/3)。HER2蛋白为阴性的患者中,HER2基因非扩增符合率为100%(73/73)。两种方法检测结果比较差异无统计学意义(P>0.05)。结论:EGFR在食管鳞癌中高表达可能与食管鳞癌的发生发展有关,并有可能成为靶向治疗的靶点,而HER2在食管鳞癌中由于表达很低,有可能不是该肿瘤分子治疗的靶点。IHC检测HER2蛋白与FISH检测HER2基因结果一致性较高。
Objective ：To explore the expressions of EGFR and HER2 in esophageal squamous cell carcinoma（ ES- CC） and their relationships with clinicopathological parameters. To investigate the conformity between the HER2 pro- tein examined by immunohistochemistry （IHC） and the HER2 gene detected with fluorescence insitu hybridization （FISH）. Methods：The expression of EGFR was examined by IHC in 76 surgically obtained ESCC specimens （group ESCC） and in 21 specimens of tumor - adjacent normal squamous epithelium as the controls（ group C）. IHC and FISH were used to detect the expression of HER2 protein and amplification of HER2 gene, respectively. Results：The ex- pressions of EGFR （63.2%） , was higher in group ESCC than those in group C （ 33.3 % , P 〈 0.05 ）. In group ESCC, HER2 expression was lower in group ESCC（3.9% ） and no expression in group C. EGFR and HER2 were correlated to vascular recidivism, lymph node metastasis. Besids, HER2 was correlated to invasion of ESCC cells. No relationship was found between HER2 and EGFR. In patients whose HER2 proteins were positive, HER2 gene amplification was found,resulted in a coincidence of 66.7% （2/3）. In whose HER2 proteins were negative, HER2 gene non -amplifi- cation was found, resulted in a coincidence of 100% （73/73）. There was no significant difference between the two methods（P 〉0.05）. Conclusion：The high expression of EGFR may correlate to tumor genesis and development of e- sophageal carcinoma and may be a new target for targeted therapy of esophageal carcinoma. The expression of HER2was low,and probably be not the target of esophageal carcinoma. There was a high coincidence between the HER2 gene examined by FISH and the HER2 protein detected with IHC.
出处
《现代肿瘤医学》
CAS
2014年第4期819-822,共4页
Journal of Modern Oncology
基金
江苏省"333"工程科研资助项目[编号:苏人才办(2009)24号]
