摘要
目的观察中药复方复健片促进脑梗死模型大鼠中枢神经再生的作用,探讨其改善运动功能的机制。方法 90只Wistar大鼠随机分为药物组、模型组、假手术组,每组30只。用电凝法制备大脑中动脉闭塞大鼠模型,于造模成功后6h药物组给予复健片水溶液9g/kg灌胃,余2组分别给予同等量生理盐水灌胃,每日1次,共2周。用横木行走实验评定运动功能;用免疫组化法观察模型大鼠梗死周边区脑组织巢蛋白(nestin)、多唾液酸神经细胞黏附分子(polysialic acid neural cell adhesion molecule,PSA-NCAM)、微管相关蛋白2(microtubule-associated protein2,MAP-2)、生长相关蛋白(growth-associated protein,GAP-43)、突触素(synaptophysin,Syn)的表达,并测定染色平均灰度或光密度值。结果 86只大鼠纳入结果分析。给药2周后药物组大鼠神经功能评分明显高于模型组,差异有统计学意义(P<0.01);药物组大鼠在梗死周边区脑组织nes-tin、PSA-NCAM、MAP-2、GAP-43、Syn各指标表达方面均较模型组明显增强,差异有统计学意义(P<0.01)。结论复健片可促进神经再生,从而改善脑梗死大鼠运动功能。
Objective To study the effects of Compound Fujian Tablet （FJT）on the neurotization in the cerebral infarction rats and to explore its mechanisms for promoting the motor skills. Methods Totally 90 Wistar rats were randomly divided into the drug group, the model group, and the sham-operation group, 30 in each group. The rat model of middle cerebral artery occlusion （MCAO）was successfully established by electrocoagulation. Six hours after successful modeling, the rats of the drug group were orally administered with 9 g/kg FJT water solution, and the other groups were orally administered with equal volume of normal saline, once a day for two weeks. The motor skills of rats were examined by beam walking test. The expressions of nestin, polysialic acid neural cell adhesion molecule （PSA-NCAM）, microtubule-associated protein 2 （ MAP-2 ）, growth-associated protein （ GAP- 43 ）, and synaptophysin （Syn） in the brain tissue around the infarction were observed by in immunohistochemical assay. The mean staining gray or the optical density value were detected. Results The 86 rats were recruited in the result analysis. After two weeks of administration, the neural function scoring was obviously higher in the drug group than in the model group with statistical difference （ P 〈0.01 ）. The expressions of nestin, PSANCAM, MAP-2, GAP-43, and Syn in the brain tissue around the infarction were more obviously enhanced in the drug group than in the model group, showing statistical difference （P 〈0.01 ）. Conclusion FJT can promote neurotization and improve the motor skill recovery after cerebral infarction.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第1期100-104,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然基金资助项目(No.30672745)
山东省科技发展计划资助项目(No.2006GG3202005)
关键词
中药复健片
脑梗死
神经再生
Compound Fujian Tablet
cerebral infarction
neurotization
