摘要
目的:优选平性人参炮制工艺。方法:采用栀子、黄连、知母分别制备平性人参;采用Bradford法测定蛋白含量;采用紫外分光光度法测定过氧化物酶(peroxydase,POD)、过氧化氢酶(catalase,CAT)和超氧化物歧化酶(superoxide dismutase,SOD)活力;采用单因素考察法,优选栀子制人参炮制工艺。结果:3种平性人参以栀子制人参抗氧化酶活力最高,其最佳炮制工艺为:物料比(人参∶栀子)1∶1,pH6.0,料液比1∶40,浸提2次,每次12 h。结论:3种平性人参均具有抗氧化酶活性,且以栀子制人参抗氧化酶活性最高;单因素试验筛选出的栀制人参炮制工艺合理、可行。
OBJECTIVE To establish the optimal processing technology of Ginseng with mild property hy the activities of peroxydase （POD）, catalasc（CAT） and superoxide dismutase（SOD）, and to establish the optimum processing technology of Ginseng with gardenia by SOD activity. METHODS The Ginseng was processed by gardenia, coptis and anemarrhena; the protein content was determined using the coomassie blue staining methods, the activities of POD, CAT and SOD were determined using the UV-visible spectrophotometry, a monofactor test was developed to determine the optimum extraction process of Ginsong with gardenia. RESULTS The activities of POD, CAT and SOD of Ginseng with gardenia were 19. 200 U.mg-1 , 120. 000 U.mg-1 and 178. 512 U.mg-1 , separately; the activities of POD,CAT and SOD of Ginseng with coptis were 13. 333 U.mg -1 , 100.000 U. mg-1 and 82. 636 U. mg-1 ; the activities of POD, CAT and SOD of Gbzseng with anemarrhena were 40. 889 U. mg -1, 17. 037 U.mg-1 and 84. 481 U.mg -1. The optimum processing technology of C-vnseng with gardenia was obtained as fol- lows： material material rate was 1：1, material-liquid rate was 1：40,extracted 12 h two times while the pH value of 6. 0 at 0. 15 mol.L-1 salinity. CONCLUSION The Ginseng with mild property that Ginseng with gardenia, coptis and anemarrhena have antioxidase activity, and the Ginseng with gardenia has the highest anti-oxidase activities. The eventually SOD activity of Gin- seng with gardenia was 320. 64 U.mg-1.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第2期115-118,共4页
Chinese Journal of Hospital Pharmacy
