摘要
为选育出有香味、观赏性好、抗性强、栽培容易的品种,以多花兰为父本、蕙兰大一品为母本进行杂交,研究人工授粉、果实生长情况,开展兰花种间杂交育种的基础研究工作,了解杂交过程中可能出现的问题;对得到的杂交种子进行非共生萌发,建立完整的非共生萌发技术体系;在杂交根状茎的培养阶段,设计不同基本培养基、激素浓度和添加物,筛选最佳根状茎增殖、分化和生根壮苗的培养基。结果表明:多花兰与蕙兰大一品杂交授粉容易,结实率高,为100%。杂交适宜采收期90～150d;接种种胚最佳萌发时间为90～150d。最佳萌发培养基为ZB11。其根状茎的最佳增殖、分化和生根培养基为:1/2MS+6-BA 0.3mg.L-1+NAA1.0mg.L-1+椰汁20mL.L-1+AC 0.5g.L-1。在试验增殖的过程中出现了较为整齐的分化和生根,分化率达到95%以上。炼苗基质以草炭∶珍珠岩∶火山石=3∶3∶4为最佳。
In order to breed out the varieties with good looking, strong resistance, fragrance, easy to cultivation, Cymbidium floribundum was used as male parent and Cymbidium foberiduse ＇ Dayipin was used as female to conduct the crossbreed interspecies. The artificial pollination and the growth of Cymbidium fruit were researched to investigate the process of the crossing. The basis research work of crossbreed of Cymbidium was carried out. The system of asymhiotic germination propagation was established rough the studies of asymotic germination of the crossbreed seeds. The appropriate culture mediums of the rhizome proliferation, differentiation and rooting were founded through the design of different basic medium, hormone concentration and additives. The results showed that it was easy to obtain hybridization pollination of Cymbidium floribundum and Cymbidium faberiduse Dayipin ,seed setting rate was 100%. Advisable harvest time was 90 - 150 d for hybrid. The best embryo germination time was 90 - 150 d. The best medium for germination was ZBH. The best proliferation, differentiation and rooting mediums of rhizomes were 1/2MS＋ 6-BA 0.3 tag. L 1 ＋ NAA 1.0 mg-L-1 ＋Coconut juice 20 mL- L 1 ＋ AC 0. 5 g- L1. In the test, the breeding process appeared relatively regular differentiation and rooting, differentiation rate was above 95%. The best base material of hardeningseedling was grass carbon： perlite： pelelith = 3 ： 3 ： 4.
出处
《黑龙江农业科学》
2013年第2期73-76,共4页
Heilongjiang Agricultural Sciences
关键词
多花兰
蕙兰
杂交
萌发
Cymbidium floribundum
Cymbidium faberiduse
crossbreed
germination
