摘要
用地生兰送春和附生兰多花兰进行种间杂交,对杂交种子进行非共生萌发和快速繁殖研究。结果表明:胚龄7个月0、.1 mol/L KOH处理10 min的种子萌发率最高;种子萌发后原球茎的增殖以Z4(KC+BA 0.4 mg/L+NAA 0.6 mg/L+KT 0.2 mg/L)培养基为最好,增殖系数可达到12.8;在原球茎增殖和分化成苗过程中加入活性碳能够有效地防止切口褐化。
Interspecific hybridization was carried out between terrestrial orchid Cymbidium cyperifolim var. szechuanicum and epiphytic orchid C. floribundum, to study on the asymbiotic germination and rapid propagation of hybrid seeds. The results showed that Seven-month-embryo-aged-seeds had the highest germination rate, when treated with 0. 1 mol/L KOH for 10 rain. After germination, the best medium for PLBs propagation was Z4 （ KC＋BA 0. 4 mg/L ＋ NAA 0. 6 mg/L＋KT 0. 2 nag/L）, the rate of PLBs proliferation could reach 12. 8. Adding activated carbon to medium prevented the cut browning in the process of PLB＇s multiplication and differentiation.
出处
《北方园艺》
CAS
北大核心
2009年第3期181-183,共3页
Northern Horticulture
关键词
杂种
非共生萌发
胚
原球茎
Hybrid
Asyrnbiotic germination
Embryo
Protocorm-like body
