摘要
目的:分析柱果绿绒蒿挥发油的化学成分并进行抗氧化活性研究。方法:采用水蒸气蒸馏法获得挥发油,运用气相色谱-质谱联用进行成分分析,同时运用紫外分光光度计测定其对羟基自由基的清除能力,用酶标仪测定其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力,并与2,6-二叔丁基-4-甲基苯酚(BHT)比较。结果:从柱果绿绒蒿的挥发油中鉴定了47个化合物,占其总量的91.866%;挥发油对羟基自由基和DPPH自由基的清除能力均强于BHT。结论:柱果绿绒蒿挥发油的化学成分复杂,主要为十六烷酸(27.653%)和6,10,14-三甲基-2-十五烷酮(16.330%);其清除自由基的能力显示出良好的抗氧化活性。
Objective： To study the chemical components of essential oils from Meconopsis oliverana and their antioxidant activ- ity. Method： The essential oil was extracted by steam distillation, and GC-MS analysis was used to identify its constituents. The OH free radical scavenging activity of the essential oils was evaluated with an enzyme mark instrument by assay of the ability of DPPH free radical scavenging. BHT was used as positive control. Result： Forty-seven compounds, account for 91. 866% of the essential oils, were identified. The ability of scavenging OH and DPPH radicals of the essential oils is stronger than that of BHT. Conclusion： The main chemical constituents of the essential oils from M. oliverana are n-hexadecanoic acid （27. 653% ） and 6,10,14-trimethyl-2-pen- tadecanone（ 16. 330% ）. And the essential oils showed strong antioxidant activity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第2期284-288,共5页
China Journal of Chinese Materia Medica
基金
西部资源生物与现代生物技术教育部重点实验室基金项目(KH09030)
西藏自治区科技厅重大科技专项基金项目(20091012)
陕西省教育厅科学研究项目(2010JK862)
关键词
柱果绿绒蒿
挥发油
化学成分
抗氧化活性
Meconopsis oliverana
essential oil
chemical constituents
antioxidant activity
