摘要
目的:应用响应曲面分析法观察归芎药对不同制法、不同配比对DPPH自由基清除率体外抗氧化活性的相互作用研究。方法:采用2,2-二苯基-1-苦肼基(DPPH)自由基清除率法测定当归-川芎10个配比,3种不同提取方法的提取物的抗氧化活性,应用非线性回归的方法确定量效曲线的各个参数,建立三维响应面模型,应用Matlab软件构建响应曲面的三维图形。结果:不同配比不同制法的30个样品,均具有明显的清除自由基活性,且表现出明显的量效关系。水提液的协同作用范围窄,几乎成点状分布,拮抗作用范围呈6条宽窄不一的条带状分布;醇提液的协同作用呈小条状分布,拮抗作用呈点状分布。在先水提后醇提液协同作用呈条状分布(3条),拮抗作用呈点状分布。总之先水提后醇提液的协同作用区域大于醇提液,水提液次之;3种提取方法大部分区域均未表现出明显协同与拮抗作用。结论:采用响应曲面分析法定量研究中药配伍药物相互作用,为相关研究提供了思路与方法的参考。
Objective： To observe the in vitro antioxidant interaction of different preparations and proportions of Danggui-Chua- nxiong drug pair in the DPPH free radical scavenging rate with the response surface methodoh）gy. Method： The 2,2-diphenyI-I-pieryl- hydrazyl （DPPH） free radical scavenging rate method was adopted for determining the antioxidant activity of extracts from Danggui- Chuanxiong with 10 proportions and three extraction processes. The response surtace methodology was used to determine the parameters of the dose-effect curve and establish a three-dimensional response surface model. The three-dimensional response surface graph was construeted with Matlab software. Result： All of the 30 samples with different proportions and preparations had antioxidant effect in scavenging free radicals and a remarkable dose-effect relationship. Their water extracts had a narrow synergistic range, with only spot distribution. Their antagonist ranges were districted in six bands of various widths. The synergistic ranges of ethanol extracts were dis- tricted in small bands, with the antagonist ranges scattered in points. The synergistic ranges of their water-alcohol extracts were distrib- uted in three bands, with their antagonist ranges scattered in points. In short, the water-alcohol extracts showed a wider synergistic range than ethanol extracts, followed by water-extracts. All of the three extraction processes showed no obvious synergistic and antago- nist effects. Conclusion： The quantitative study on the interaction of traditional Chinese medicines with different compatibilities with the response suffaee methodology provides reference of thoughts and methods for relevant studies.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第2期234-238,共5页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目(2008BAI51B01)
高等学校博士学科点专项科研基金(20113237110010)
江苏省高校自然科学重大基础研究项目(06KJA36022
10KJA360039)
国家教育部"新世纪优秀人才支持计划"(NCET-09-0163)
江苏高等学校优秀科技创新团队支持计划(2009年度)
江苏高校优势学科建设工程项目(ysxk-2010)
关键词
当归
川芎
药对
抗氧化
DPPH
响应曲面分析法
相互作用
Danggui
Chuanxiong
drug pair
antioxidant effect
DPPH
response surface methodology
interaction
