摘要
采用柱前衍生反相高效液相色谱法对不同颜色(白、绿)、粗细和部位(尖部、中部、根部)的芦笋中游离氨基酸含量进行测定,并采用非参数检验法分析其对游离氨基酸含量的影响。结果表明:在不同芦笋段中能检测出至少15种游离氨基酸,且含量存在差异;所测样品中两种酰胺Gln和Asn含量最高;两种酸性氨基酸Glu和Asp含量次之;Cys2、Trp、Leu和Ile含量最少,Met未能检测到。芦笋的不同颜色、粗细和部位对某些氨基酸含量有显著影响。
This work presents a reversed phase high-performance liquid chromatography （RP-HPLC） method for the determination of free amino acids in asparagus after precolumn derivatization. The effects of different colors, thicknesses and parts on free amino acid contents in asparagus were investigated by non-parametric tests. At least 15 free amino acids with were detected and their contents showed differences among three different parts of asparagus （bottom, middle and top）. All investigated samples were highest in asparagine and glutamine, glutamic acid and aspartic acid were in the middle, the contents of cysteine, trytophan, leucine and isoleucine were the lowest, and methionine was not detected. Different colors, thicknesses and parts had significant effects on free amino acid contents in asparagus.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第1期65-68,共4页
Food Science
基金
国家质检总局科技计划项目(2010IK157)
关键词
芦笋
游离氨基酸
颜色
粗细
部位
asparagus
free aminoacid
color
thickness
part
