摘要
环氧水解酶能应用于外消旋环氧化物的动力学拆分或对映归一性水解制备光学纯的环氧或邻位二醇,具有广阔的应用前景。近年来,多个环氧水解酶晶体结构的报道使人们对它的结构基础有了更深入的理解。随着基因信息的增长,分子生物学和蛋白质工程技术的发展大大简化了大量克隆表达多样性环氧水解酶的过程,降低了环氧水解酶分子改造的难度,为新型具有工业应用潜力的环氧水解酶的开发提供了技术支持。本文综述了环氧水解酶的结构与机制以及近年来环氧水解酶重组表达及分子改造的研究进展。
Epoxide hydrolases （EHs） were widely used in the synthesis of chiral epoxides and diols by kinetics resolution or enantioconvergent hydrolysis. Recently, with the crystal structures of EHs, more details about their catalytic mechanism were obtained. With the development of bioinformatic, molecular biology and protein engineering, more EHs were cloned, expressed and engineered. This review focused mainly on the structure basis of EHs and the development of novel EHs as biocatalyst.
出处
《生物加工过程》
CAS
CSCD
2013年第1期77-86,共10页
Chinese Journal of Bioprocess Engineering
基金
国家重点基础研究发展计划(973计划)资助(2011CB710803)
关键词
手性合成
环氧水解酶
结构基础
分子生物学
chiral synthesis
epoxide hydrolase
structure basis
molecular biology
