摘要
利用绿豆环氧水解酶催化(R,S)-对硝基苯乙烯氧化物水解,产物(R)-对硝基苯乙二醇的产率超过常规酶促拆分的极限产率(50%),表明发生了对映体会聚.采用绿豆粉剂和粗酶制剂考察了不同条件下的生物催化反应,结果表明,添加吐温-80作为分散剂对反应有促进作用.对该酶的固定化进行了初步研究,发现酶经硅藻土吸附固定化后稳定性显著增加.在克级规模制备实验中,产物的总产率达73.3%;产物经一步重结晶,得到了光学纯度大于99%的(R)-对硝基苯乙二醇.
Mung bean epoxide hydrolase was used for enzymatic preparation of （R）-p-nitrophenyl glycol by enantioconvergent hydrolysis of p-nitrostyrene oxide. Enzyme forms and reaction conditions were examined, and an efficient process and high product yield were obtained for this biocatalyst. Tween-80 was found to be an ideal dispersant. Various immobilized biocatalysts were prepared by different methods, and the biocatalyst adsorbed on diatomite powder showed better stability. Gram-scale preparation was performed, giving a yield of 73,3 % and an optical purity higher than 99 % ee,
出处
《催化学报》
SCIE
CAS
CSCD
北大核心
2008年第8期696-700,共5页
基金
国家自然科学基金(20576037
20672037)
