摘要
目的:建立五味子配方颗粒的HPLC指纹图谱,为科学评价其质量提供依据。方法:采用高效液相色谱法,以Waters Xbridge C18柱(4.6 mm×250 mm,5μm)为色谱柱,以乙腈-水为流动相进行梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,柱温25℃。结果:建立了五味子配方颗粒的指纹图谱,确定了18个共有峰,各批五味子配方颗粒样品指纹图谱与对照指纹图谱相似度均>0.9,可用于五味子配方颗粒的质量控制。结论:该方法简单、准确、重复性好,能有效地对五味子配方颗粒的质量进行评价。
Objective： To establish a method of fingerprint analysis on S. chinensis Formula Granules by HPLC and provide a reference for quality evaluation of S. chinensis Formula Granules. Method： The chromatographic fingerprints were obtained by the Waters Xbridge Cls column （4.6 mm ×250 mm, 5 μm） with the gradient elution solvent system composed of acetonitrile-water. The detective wavelength was set at 254 nm. The flow rate was 1.0 mL .min-1 and the column temperature was maintained at 25℃. Result： 18 co-possessing peaks were selected as the fingerprint peaks of S. chinensis Formula Granules and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of S. chinensis Formula Granules and the standard fingerprint. Conclusion： The method with good reproducibility is simple and accurate. It can be used as a quality control method for S. chinensis Formula Granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期81-84,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省教育部科技部产学研结合项目(2011A091000005)
关键词
五味子配方颗粒
HPLC
指纹图谱
Schisandra chinensis Formula Granules
HPLC
fingerprints
