摘要
目的:建立紫花大叶柴胡柴胡皂苷a的含量测定方法。方法:色谱条件Phenomenex luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(74∶26)为流动相,流速0.9 mL.min-1,柱温35℃,检测波长200 nm。结果:柴胡皂苷a进样量在0.11～4.4μg与峰面积线性关系良好(r=0.999 9)。柴胡皂苷a平均回收率为97.42%,RSD 1.42%。结论:紫花大叶柴胡中柴胡皂苷a的HPLC梯度洗脱法灵敏、准确,专属性强,重复性好。
Objective： To establish a method for the determination saikosaponin a from Bupleurum longiradiatum var. porphyranthum. Method： HPLC conditions： Phenomenex luna C18 column （4.6 mm ×250 mm, 5 μm） with methanol-water （74： 26） as mobile phase ; flow rate was 0. 9 mL min-1 ; column temperature was set at 35℃ , and the detection wavelength was at 200 nm. Result： Saikosaponin a was linear in the range of 0.11-4.4 μg （r = 0.999 9） .The average recovery was 97.42% with RSD of 1.42%. Conclusion： HPLC gradient elution method for determination of saikosaponin a from B. longiradiatum var. porphyranthum is sensitive, accurate, specific, and reproducible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期71-73,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆科技重大专项计划(CSTC
2010AC5171)
关键词
紫花大叶柴胡
HPLC
柴胡皂苷A
Bupleurum longiradiatum var. porphyranthum
HPLC
saikosaponin a
