摘要
目的:建立高效液相色谱法测定山柰药材中山柰素的含量,并对不同产地山柰药材中山柰素含量进行分析,为评价药材质量提供参考。方法:使用ODS2 C18色谱柱(4.6 mm×150 mm,5μm)。流动相甲醇-0.4%磷酸(50∶50),流速1 m L·min-1,柱温室温,检测波长367 nm,进样量为10μL。结果:山柰素在0.015 8~1.58μg线性关系良好,加样回收率为97.8%,RSD 1.6%。结论:不同产地山柰中山柰素的含量差异较大,所建立的含量测定方法具有操作简单、结果准确、重复性较好等特点,可用于山柰药材质量控制。
Objective： To establish HPLC method for the determination of kaempferol in Kaempferiae Rhizoma,analyze the contents of kaempferol in Kaempferiae Rhizoma from different origins,and provide reference for the evaluation of the medicinal quality. Method： ODS2 C18（4. 6 mm × 150 mm,5 μm） was ised as chromatographic column,with methanol-0. 4% phosphate（50∶ 50） as the mobile phase; the flow rate was 1 m L·min-1,column temperature was room temperature; the detection wavelength was set at 367 nm; and the injection volume was 10 μL. Result： Kaempferol showed good linear relationship in the concentration range of 0. 015 8-1. 58 μg; the recovery rate was 97. 8%,RSD of 1. 6%. Conclusion： There was great difference in content of kaempferol in Kaempferiae Rhizoma from different origins. The established method is simple,accurate,repeatable,and can be used for quality control of Kaempferiae Rhizoma.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第3期35-38,共4页
Chinese Journal of Experimental Traditional Medical Formulae
