摘要
本文以1-脱氧野尻霉素(DNJ)的含量和提取率为考察指标,对使用AB-8大孔树脂和732H型阳离子树脂分离纯化桑叶中DNJ的工艺进行了研究。使用AB-8大孔树脂对提取液进行脱色除杂处理,上样液pH值为4.0,上样量不超过2 BV,流速为1 BV/h,可将提取液中DNJ含量从0.23%提高到4.9%,回收率为98%。采用732H型阳离子树脂层析法进一步纯化,分段洗脱流程为:1 BV去离子水-1.5 BV0.3 M氨水-2 BV0.5 M氨水,洗脱流速为2 BV/h,当流出液pH为9～10时,DNJ被洗出。产品中DNJ含量为16.7%,此工艺纯化效果好,方法简单,能够为工业化生产高浓度DNJ提取物提供基础工艺数据。
This paper studied the technology for isolation and purification of 1-Deoxynojirirnycin （DNJ） from Morus alba L. leaves by using AB-8 macroporous resin and 732 H type cation exchange resin sequentially, with the content and recovery rate of DNJ as indexes. The result showed that AB-8 resin was suitable for the preliminary isolation and purification of DNJ. At pH 4.0, the maximum loaded amount of Morus alba L. leaves extract was 2BV and the recovery rate of DNJ was 98% by collecting the solution flowing out at 1BV/h as flowing rate. The content of DNJ was enhanced to 4.9%. 732H type cation exchange resin chromatography was used to purify continuously. Elution order is ： 1 BV H20-1.5 BV 0.3 M NH3-2BV 0.5 M NH3. The final output was obtained by collecting the fraction with pH 9.0-10.0. The final total DNJ content was 16.7%. The technology is suitable for large scale manufactory of extract rich of DNJ.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第1期167-169,180,共4页
Modern Food Science and Technology
关键词
桑叶
1-脱氧野尻霉素
大孔树脂
阳离子树脂
纯化
Morus alba L. leaves
1-Deoxynojirimycin
macroporous resin
cation exchange resin
purification
