摘要
目的:观察牛磺胆酸(Taurocholate Acid,TCA)对小鼠腹腔巨噬细胞钙调素(calmodulin,CaM)基因表达的影响,探讨TCA对小鼠腹腔巨噬细胞的作用机制。方法:分离培养小鼠腹腔巨噬细胞,应用荧光定量RT-PCR技术检测TCA对小鼠腹腔巨噬细胞CaM mRNA表达的影响。结果:与LPS刺激组相比较,高剂量(15μg/mL)和低剂量(0.15μg/mL)的TCA组小鼠腹腔巨噬细胞CaM mRNA的表达均显著高于LPS刺激组(P<0.05)。结论:TCA能显著促进小鼠腹腔巨噬细胞CaM mRNA的表达。
Objective： To observe the effect of Taurocholate Acid（TCA） on calmodulin（CaM） expression in peritoneal macrophage in mice and to explore its effective mechanism.Methods： The peritoneal macrophages of mouse were isolated and cultured.The effects of TCA on the expression of CaM mRNA was examined quantitatively by real-time fluorescent quantitative RT-PCR.Results： TCA（15μg/mL and 0.15μg/mL） decreased significantly the expression of CaM mRNA in peritoneal macrophage in mice（P 0.05）,compared with that in the LPS group.Conclusion： TCA can significantly promoted the expression of CaM mRNA in peritoneal macrophage in mice.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2012年第3期1-4,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然基金资助(No 31160518)
关键词
牛磺胆酸
钙调素
荧光定量RT-PCR
腹腔巨噬细胞
Taurocholate Acid
Calmodulin
Real-time Fluorescent Quantitative RT-PCR
Peritoneal macrophage
