摘要
研究旨在对红酵母D(Rhodotorula sp.D)利用玉米浆发酵生产含类胡萝卜素功能饲料的培养基的碳氮源进行优化,并进行工业化生产试验。对玉米浆和葡萄糖结晶废糖液分别进行单因素试验来确定最佳碳氮源比例,进行7 L发酵罐试验确定上罐参数,1 000 L发酵罐中试并对发酵液进行喷雾干燥制取产品。结果表明葡萄糖结晶废糖液和玉米浆的添加量分别为7%和33.3%,7 L发酵罐最佳发酵时间为72 h,此时的生物量、色素含量和色素产量分别达到30.2 g/l,374.1μg/g和11.3 mg/l;1 000 L最佳发酵时间为41 h,此时的菌体生物量,色素含量,色素产量分别为42 g/l,404.6μg/g,17 mg/l。喷雾干燥后的产品粗蛋白质含量达到31%,类胡萝卜素含量为180.4μg/g。结果表示,优化培养基组分,控制好发酵条件,就可以利用玉米浆发酵生产富含类胡萝卜素红酵母增色功能饲料。
The purpose of this study is to optimize the ratio of carbon and nitrogen sources for the fermentative production of functional feed in rich of carotenoid by rhodotorula sp.D in laboratory scale and then for industrial scale test. Single factor tests were performed to determine the opti- mum proportion of corn steep liquor （nitrogen source） and waste sugar water （carbon source） in the 7 L fermentor. The parameters were then re-tested in a 1 000 L fermentor. The final products were spray dried from the fermentation liquid. The results show that the favorable amounts of waste sug- ar water and corn steep liquor were 7% and 33.3% for laboratory scale test. Within 72 h fermenta- tion, the biomass, carotenoid content and carotenoid yields reached 30.2 g/1,374.1 μg/g and 11.3mag/l, respectively; The fermentation time for the 1 000 L fermentor is 41 h, with the biomass, carotenoid content and carotenoid yields as 42 g/1,404.6 μg/g and 17 mg/l, respectively. After spray drying, there are 31% of the crude protein content and 180.4 μg/g of carotenoid content in the final prod- ucts. The results suggest us an optimized ratio of medium components and favorable fermentation conditions for production of functional feed in rich of carotenoids with corn steep liquor.
出处
《饲料工业》
北大核心
2013年第1期34-37,共4页
Feed Industry
关键词
类胡萝卜素
饲料
玉米浆
红酵母
carotenoid
feed
corn steep liquor
rhodotorula
