摘要
目的:分析microRNA-16(miR-16)在系统性红斑狼疮(SLE)患者血浆中的表达水平及其临床意义。方法:用茎环RT实时荧光定量PCR(RT-qPCR)检测48例SLE患者和20例健康人血浆中miR-16的表达水平,并分析其与SLE各种临床资料的相关性。结果:茎环RT-PCR可以特异性扩增血浆中的miR-16。miR-16在SLE患者血浆中表达水平下调,和健康人相比差异有统计学意义(其Ct值分别为19.40±0.85和18.89±0.66,t=2.386,P=0.02)。血浆miR-16表达水平与SLE患者活动程度的SLEDIA评分呈负相关(r=0.313,P=0.03),SLE疾病活动度越高,miR-16水平越低。结论:SLE患者血浆miR-16下调表达与SLE疾病活动度有关。
Objective： To analyze the expression and clinical significance of microRNA-16 （miR-16） in the plasma of patients with systemic lupus erythematosus （SLE）. Methods： Using Stem-loop RT real-time polymerase chain reaction （RT-qPCR） based on the SYBR Green I, the expression of plasma miR-16 in 48 cases of SLE patients and 20 healthy controls were detected. The expression levels of plasma miR-16 were correlated with commonly used clinicopathologic features of SLE. Results： Stem-loop RT-qPCR could specifically amplify miR-16 in plasma. The expression of plasma miR-16 was significantly decreased in the patients with SLE compared with healthy controls （t=2.386, P=0.02）. Their Ct values were 19.40 ＋ 0.85 and 18.89 ＋ 0.66 respectively. Moreover, plasma miR-16 expression levels was negatively correlated with SLEDIA score （r=0.313, P=0.03）. The higher disease activity of SLE patients had the lower level of miR-16 expression in plasma. Conclusion： Down-regulated expression of miR- 16 in the plasma may play an important role in the development and progression of SLE disease.
出处
《温州医学院学报》
CAS
2013年第1期26-30,共5页
Journal of Wenzhou Medical College
基金
浙江省科技厅科研基金资助项目(2012C33126)
温州市科技局科研基金资助项目(Y20100287)
浙江省大学生科技创新活动计划(新苗人才计划)资助项目(2012R413051)
温州医学院学生科研立项资助项目(WYX201201076)
