摘要
目的研究和分析应用血浆检测乙肝两对半效果。方法采用ELISA方法,在试剂的酶标记物中加入10%吐温50ul后,检测血浆乙肝两对半。结果血浆检测乙肝两对半不加入吐温的检出率和加吐温的检出率HBsAg、HBsAb和HBeAg这3项与血清相同,但酶标物加入50ul10%吐温后检测血浆乙肝两对半较不加吐温的检出率HBeAb和HBcAb从89.70%和91.17%提高到98.53%和100%,与血清的检出率基本相同。结论应用血浆检测乙肝两对半是可行的。
Objective To search and analyse the effect of using plasma to detect hepatitis B. Methods ELISA method was used. A.fter 50ul 10% tween being added to enzyme labeled compound in the reagent, plasma hepatitis B were detected. Resuits Plasma was used to detect hepatitis B, the detectable rate of tween not being added and being added, the three items HBsAg,HbsAb and HbcAg were the same as blood serum. But after 50ul 10% tween was added to enzyme labeled compound, plasma was used to detect hepatitis B and the detectable rate HBeAb and HbcAb increased from 89.7% and 91.17% to 98.53% and 100%. It was the same as blood serum on the whole. Conclusion Blood plasma being used to detect hepatitis B is feasible.
出处
《中国现代医药杂志》
2006年第2期13-14,共2页
Modern Medicine Journal of China
