摘要
为快速繁殖扁桃，以从美国引种的5个品种为试材，从取材、材料的处理、外植体的诱导分化、芽的增殖、生根以及影响试管苗形成的重要因子等方面探讨了扁桃组织培养的技术和方法。经研究得出生长素与细胞分裂素对芽的诱导分化及芽的增殖最佳配比浓度为：在改良MS基本培养基上添加0.5mg/L的NAA，0.5mg/L的KT，0.1mg/L的BA，此配比浓度产生的扁桃有效苗最多；而添加1.5～2.0mg/L的IBA生长素有利于扁桃组培苗生根。
Five varieties of Prunus communis Frish introduced from America as samples, the study on techniques and measures o f tissue culture of Prunus communis Frish stem apex was carrie d out from aspects of sample preparing, explant differentiation inducting, bud p ropagating, root generating and factors affecting test-tube plantlet formation. The results show that the optimum medium for inducing bud differentiation is to add 05mg/L NAA, 05mg/L KT and 01mg/L BA to the improved MS medium, while a dding 15～20mg/L IBA to the medium is favorable to root generation of tissue culture plantlets.
出处
《云南林业科技》
2000年第2期24-28,共5页
Yunnan Forestry Science and Technology
基金
云南省"九五"攻关课题
