摘要
目的考察板蓝根不同部位提取物,即30%醇沉多糖I、50%醇沉多糖II、70%醇沉多糖III,及板蓝根水提取液的大孔树脂水洗脱部位IV、10%醇洗脱部位V、30%醇洗脱部位VI、50%醇洗脱部位VII,以及分得的单体落叶松脂素-4,4'-二-O-βD-二葡萄糖苷(CB)VIII、异落叶松脂醇IX的抗甲型流感病毒A/PR8/34(H1N1)活性。方法采用抗甲型流感病毒A/PR8/34(H1N1)体内实验,通过计算肺指数、肺指数抑制率等,综合检测板蓝根提取部位及单体化合物的抗甲型流感病毒作用。结果 II、III、IV、V、VI、VII、VIII及IX可明显延长甲型流感病毒感染小鼠存活天数,与病毒模型组比较,具有显著性差异(P<0.05),II、III、IV、V、VI、VII、VIII及IX可减少感染小鼠死亡率;同时II、VI、VII及单体VIII可明显降低肺指数(P<0.05),肺指数抑制率分别为31.4%、28.5%、30.5%和34.0%。结论板蓝根有效组分II-IX对甲型流感病毒感染小鼠具有较好的保护作用,提示其中化合物CB可作为抗病毒的主要有效及指标性成分。
OBJECTIVE To study the antiviral activities of 30M EtOH-sinked polysaccharide I, 50% EtOH-sinked polysac- charide II and 70% EtOH-sinked polysaccharide IIl which are extracts of different parts of Radix Isatidis, D101 macroporous resin water-eluted part IV, 10M EtOH-eluted part V, 30% EtOH-eluted part VI, 50% EtOH-eluted part VII which from the water extract of Radix Isatidis, and compound slariciresinol-4, 4＇-bis-O-β-D-glucopyranoside （CB） VIII and isolariciresino IX on influenza A virus A/PRS/34（HIN1）.METHODS The anti-influenza A virus A/PR8/34（HIN1） in vivo experiment was applied. And the antiviral activities of extract parts and monomeric compounds were tested by measuring lung index and !ung index inhibition rate. RESULTS II, III, IV, V, VI, VII, VIII and IX could obviously prolong the living time of mice infected by influenza A virus, and the results showed marked differences compared with control group （P〈0.05）. II, III, IV, V, VI, VII, VIII and IX could decrease the death rate of infected mice; at the meantime, II, VI, VII and VIII could also obviously re- duce lung index （P^0.05）, and the lung index inhibition rates were 31.4 %, 28.5 %, 30.5%, and 34.0% respectively. CON- CLUSION The extract II-IX of Radix Isatidis can protect the mice infected by influenza A virus, and CB may be the effective and index component with antJviral activity in Radix Isatidis. KEY WORDS; Radix Isatidis; antivirus; activity in vivo; lung index
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2013年第1期53-55,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金(81073023)
国家教育部博士点基金(20093237120015)
江苏省高校自然科学基金(09KJB360006)
江苏省产学研联合创新基金(BY2009109)
江苏高校优势学科建设工程资助项目(ysxk-2010)
关键词
板蓝根
抗病毒
体内活性
肺指数
Radix Isatidis
antivirus
activity in vivo
lung index
