摘要
采用酸性聚丙烯酰胺凝胶电泳 (APAGE)对 1998至 1999年四川省区试的 2 4个参试品系和两个对照品种进行了醇溶蛋白位点的特异性检测。结果表明 :2 6个材料共出现 2 2种带型。每个材料可分离出 15~ 2 1条谱带 ,共分离出 34条带 ,其中 2 7条具多态性 ,占 79 4%。 2 4个品系中 ,有 2 0个具有 1B/ 1R易位标记位点Gld1B3 ,占供试材料的 83 3 %。
Gliadin variations of 26 advanced lines of Sichuan bred in 1998~1999 were investigated by acid polyacrylamide gel electrophoresis (APAGE). Thirty-four gliadin bands are seperated by electrophoresis and 27 out of them show polymorphism (79.4%). Each advanced line has 15 to 21 bands. Twenty out of 24 lines (83.3%) has the allele Gld1B3. These rusults suggest that the advanced lines in Sichuan exhibit higher gliadin genetic diversity.
出处
《四川农业大学学报》
CSCD
2000年第1期21-24,共4页
Journal of Sichuan Agricultural University
基金
国家自然科学基金资助! (批准号 :39870 5 47)