摘要
以含有Yr9的抗条锈病近等基因系Taichung29*6/Yr9及其轮回亲本Taichung29为材料,用目的基因所在1B染色体上32对微卫星引物对其基因组DNA进行PCR扩增,发现引物Xgwm582在近等基因系与轮回亲本间可扩增出特异性DNA片段。经F2代分离群体177个抗、感单株检测证实,该片段位点与抗条锈病基因Yr9紧密连锁,遗传距离为3·7cM,确定Xgwm582可作为抗条锈病基因Yr9的标记。
SSR analysis was performed using a wheat near-isogenic line（NIL） Taichuang29 ＊ 6/ Lovrin13,which carried the resistance gene Yr9 against wheat stripe rust and its recurrent parent Taichung29 as materials. After screening with 32 SSR primers on 1 B chromosome, reproducible polymorphic DNA fragment amplified by Xgwm582 was identified. Genetic linkage was tested in 177 segregating F2 plants. The results indicated that microsatellite marker Xgwm582 was linked with gene Yr9 resistant to wheat stripe rust. A genetic distance of 3. 7 cM was calculated.
基金
国家"973"计划项目(编号:G2000016200)
国家"863"计划项目(编号:2002AA245041)
国家自然科学基金项目(编号:30471131
30270867)~~
关键词
小麦
抗条锈病基因
Yr9
微卫星标记
wheat
resistance gene against stripe rust
Yr9
microsatellite marker
