摘要
以一品红27个品种为试材,探讨其单药花粉量及其花粉离体萌发特性差异;并从中选择花粉相对活力低、高组4个品种("双喜"和"威望"、"金奖"和"金多利")的花粉,以花粉离体萌发法为对照,对I-KI染色法、TTC染色法、过氧化物酶染色法3种染色测定花粉活力法进行比较筛选。结果表明:测试品种单药花粉量变化范围为817～3 867粒,花粉萌发率18.04%～82.07%,花粉管长度0.20～1.52 mm;品种间存在明显差异。花粉量、花粉萌发率、花粉管长度3指标间极显著正相关。对上述各因子聚类分析表明供试品种中可萌发花粉量(花粉量×花粉萌发率)少且花粉管生长速度慢的品种相对较多,占40.74%。过氧化物酶染色法测定值与对照不存在显著性差异,可用于一品红花粉活力快速测定。
Variation analysis for the quantity and germination characteristics of pollens was carried out in vitro on 27 Euphorbia pulcherrima cultivars.‘Carri Red’ and ‘Prestige Red’,‘Premium Red’ and ‘Capri White’ were chosen as the experimental materials for determining their different pollen viability.Comparison of three testing methods,including TTC coloration,I-KI coloration,peroxide enzyme coloration,were used.The result showed that significant variations in the pollen quantity,germination rate and length of the pollen tubes were observed among the different cultivars,which were estimated to be 817-3867 grains per anther,18.04%～82.07% and 0.20-1.52 mm respectively.There was significant positive correlation among the pollen quantity,germination rate and length of the pollen tubes.Further investigation on the pollination value of different E.pulcherrima cultivars was performed based on cluster analysis on the above data.The population of the cultivars with low germinated pollen quantity and shorter pollen tube length was relatively larger,accounting 40.74% of the total tested cultivars.The statistic data from peroxide enzyme coloration differed little from the germination rate testing method,so it is believed this method may be the better one for testing E.pulcherrima pollen viability.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第6期1130-1135,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
江苏省农业科技自主创新资金项目(cx(10)411)
关键词
一品红
花粉量
花粉萌发率
花粉管长度
聚类分析
Euphorbia pulcherrima
pollen quantity
pollen germination rate
pollen tube length
cluster analysis
