摘要
目的探讨绞股蓝对血管性痴呆(VD)小鼠认知功能障碍和海马神经元损伤的保护作用。方法随机将雄性C57BL6/G小鼠分为假手术(Sham)组、血管性痴呆模型(VD)组、绞股蓝治疗组(低、中、高剂量组)。采用双侧颈总动脉阻断(2VO)方法复制血管性痴呆小鼠模型,Morris水迷宫检测小鼠的学习记忆能力,HE染色法观察小鼠海马神经元的形态学变化。结果 Morris水迷宫测试发现VD小鼠学习记忆能力下降,海马CA1区神经元迟发性死亡;绞股蓝治疗可显著提高VD小鼠的学习记忆能力,减少VD所致的海马CA1区神经元死亡;中、高剂量绞股蓝治疗组VD小鼠逃避潜伏期的时间明显短于低剂量组,穿越平台次数明显多于低剂量组,其差异具有统计学意义(P<0.05)。结论绞股蓝可通过保护血管性痴呆所致的小鼠海马神经元损伤,改善VD小鼠的认知功能。
Objective To investigate the neuroprotective effects of gynostemma on vascular dementia - induced cognitive dysfunction and neuronal injury in mouse hippocampal CA1 region. Methods C57BL/ 6 mice were randomly divided into sham - operation group （ control ）, the vascular dementia （VD） group and gynostemma- treated （low, medium and high dose） groups. The mouse vascular dementia model was induced by bilateral common carotid artery occlusion （2VO）. Morris water maze was used to measure mouse learning and memory ability. The neuronal injure in mouse hippocampal CA1 region was detected by HE staining. Results The Morris water maze test showed that vascular dementia induced the decreased spatial learning and memory ability and the delayed neuronal death in hippocampal CA1 re- gion. Gynostemma treatment significantly improved cognitive dysfunction and reduced neuronal death in hippocampal CA1 region of VD mice. In addition, compared with the low dose of gynostemma group, the reduced escape latency and the increased times of crossing the platform were detected in high and medium doses of gynostemma groups. Conclusion Gynostemma could produce neuroprotective effects a- gainst VD -induced mouse cognitive dysfunction and neuronal injury.
出处
《合肥医学院学报》
2012年第5期371-374,共4页
Journal of Zunyi Medical University
基金
贵州省科学技术基金资助项目(NO:J20122351)
合肥医学院重点学科建设经费资助项目(NO:20121602)
关键词
绞股蓝
血管性痴呆
认知功能
小鼠
gynostemma
vascular dementia
cognitive function
mouse
