摘要
对提取β-胡萝卜素后的杜氏盐藻渣中多糖的提取、初步纯化和多糖脱色方法进行了实验研究.实验采用水浸提的方法,考察了提取温度、提取时间、液固比和pH值对盐藻多糖提取率的影响,盐藻多糖的最适提取条件为:提取温度:70℃,提取时间:4 h,液固比:30:1,pH值10,在此条件下,盐藻多糖的提取率为8.63%,多糖含量为65.73%.采用分级醇沉和分步醇沉的方法沉淀多糖,得到醇沉最佳条件为无水乙醇一步醇沉.采用中性蛋白酶水解脱除盐藻多糖提取液中蛋白质,脱除率为61.55%,脱蛋白后多糖含量为70.96%,采用过氧化氢对脱蛋白后多糖提取液脱色,脱色率为78.66%.
The extraction technology,primary purity method and decolorization method of polysaccharide from Dunaliella salina residue after β-carotene are extracted.A water immersion extraction method was adopted in this experiment,the effects of extraction temperature,extraction time,material to liquid ratio,pH value on extraction efficiency of D.salina polysaccharide were investigated.The optimal extraction condition was extraction temperature 70℃,extraction time 4 h,material to liquid ratio 30 ml/g,pH 10,under this condition,the extraction ratio was 8.63% and the polysaccharide concentration was 65.73%.The optimal precipitation condition of polysaccharide was adopted the way of once alcohol-precipitation by 100% ethanol（v/v）.The deproteinizing ratio of polysaccharide extracts was 61.55% after hydrolyzed by neutral protease,its polysaccharide content is 70.96%,and the decolorization ratio was 78.66% by hydrogen peroxide.
出处
《渤海大学学报（自然科学版）》
CAS
2012年第4期334-340,349,共8页
Journal of Bohai University:Natural Science Edition
关键词
盐藻
多糖
提取
醇沉
脱色
Dunaliella salina
polysaccharide
extraction
alcohol-precipitation
decolorization
