摘要
目的:研究肉桂对人宫颈癌细胞生长的影响。方法:肉桂水煎液作用于宫颈癌细胞后,CCK-8法检测细胞增殖和贴壁情况,划痕实验分析细胞的迁移能力,流式细胞术分析细胞周期。结果:作用48h后,高、中、低浓度肉桂组宫颈癌细胞生长抑制率分别为97.12%、24.58%、15.40%;贴壁率为48.3%,与对照组相比明显减少(P<0.05),且明显降低细胞的迁移(P<0.05);FCM显示细胞周期移行被阻滞于G2期。结论:肉桂对体外人宫颈癌细胞增殖有明显抑制作用,并可降低肿瘤细胞贴壁率和迁移能力,阻滞细胞周期于G2期。
Objective：To investigate the effects of cinnamon on the growth of Hela cells.Method：Hela cells were treated with the decoction of cinnamon,and its effects on the proliferation and adhesion of Hela cells were determined by the CCK-8 assay.The Scratch Test and Flow Cytometry were used to analyze Hela cells’ migration and growing cycle.Result：After 48 hours, cinnamon group cell growth inhibition rate respectively was 97.12%,24.58%,15.40%,which measure in high,middle and low concentration.Comparing with the control group,the adherence rate was 48.3%（P0.05）and the rate of Hela cells’ migration was reduced significantly（P0.05）.FCM showed that the cell cycle was arrested at G2 phase.Conclusion：Cinnamon can inhibit Hela cells in vitro and may be block the growth, Conglutination, movement and infiltration of Hela cells.
出处
《中国医学创新》
CAS
2013年第1期13-14,共2页
Medical Innovation of China
基金
广东省中医药局科研项目(编号:20121113)
高等学校特色专业建设点经费(编号:TS10466)
