摘要
以粳稻Azucena为父本与舢稻IR64杂交发展的一双单倍体（DH）群体，与籼稻IR1552杂交发展的一重组自交系（RI）群体为材料，应用分子标记图谱对2个群体在大田和盆栽2个环境下的穗长进行QTLs及上位性效应分析。DH群体中共检测到6个穗长QTLs，位于第1、4条染色体上的3个QTLs，在2个环境中稳定表达，未检测到上位性效应，加性效应为穗长遗传主效应。R1群体中，共检测到3个穗长QTLs及6对互作效应位点，位于策4条染色体上的1个QTL及位于第1、12条染色体上的2个互作位点在2个环境中稳定表达，上位性效应表现为遗传主效应。在2个群体中均检测到的与穗长相关的1个QTL位于第4条染色体RG163～RZ23区间内，遗传正效应等位基因来源于Azucena；位于第1条染色体与RG323连锁的位点在DH群体中表现为1个QTL，但在RI群体中表现为互作位点。
A double haploid (DH) population and a recombinant inbred (RI) population derived from a cross between a japonica male parent Azucena and indica female parents, IR64 and IR1552 respectively, were used in both field and pot experiments for detecting QTLs and epistasis for rice panicle length in different genetic background and different environments. Panicale length (PL) was measured at maturity. QTLs for PL were detected using single marker analysis and interval mapping. Epistasis effects on the trait were also analyzed. Nine QTLs were detected in DH population, including 5 QTLs detected from field experiment and 4 from pot experiment among them 3 QTLs mapped on chromosomes 1 and 4 were identified in both field and pot experiments. No significant epistasis effect was detected for PL Four QTLs were detected in RI population, among them 2 from field condition and 2 from pot experiment. 6 pairs of epistasis loci were detected in RI population. One QTL mapped on chromosome 4 and two pairs of epistatic loci were detected in both field and pot experiments. One QTL on chromosome four was identified in both populations, and one marker locus RG23 on chromosome one was involved in additive effect in DH population, but epistasis effects in RI population.
基金
国家自然科学基金
关键词
水稻
穗长
QTLS
上位性
遗传背景
环境条件
育种
rice (Oryza sativa L.), panicle length
QTLs
epistasis
RFLP marker, AFLP marker
