摘要
本实验通过双向凝胶电泳技术(2-DE)比较橡胶死皮树与健康树胶乳黄色体蛋白表达谱的差异,通过软件分析比较获得37个差异表达的蛋白点,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后,搜索NCBInr数据库,结果表明,有11个蛋白点被成功鉴定,其中7个为未知蛋白,其余4个蛋白分别为chloroplast 23 kDa polypeptide of photosystem II、ATP synthase beta chain、NADP-dependent sorbitol-6-phosphate dehydrogenase和vitamin-b12 independent methionine synthase。这4个蛋白在死皮树中表达量均下调,可能在死皮发生过程中起着重要作用。本研究将为进一步阐明橡胶树死皮发生的分子机制提供理论依据。
In this experiment, the two-dimensional gel electrophoresis（2-DE） was used to study the differentiation of protein expression profiles of latex lutoid between TPD trees and healthy ones in Hevea brasiliensis. 37 differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser deserption/ionization time of flight mass spectrometry （ MALDI-TOF MS） followed by searching for NCBInr database, the result showed that 11 spots were successfully identified, of which 7 were identified as the unknown proteins, and the remaining 4 were the chloroplast 23 kDa polypeptide of photosystem II, ATP synthase beta chain, NADP-dependent sorbitol-6-phosphate dehydrogenase and vitamin-bl2 independent methionine synthase. There were some relationship between osmotin protein and TPD broom according to the functions of down-regulated proteins in TPD trees. This research provided a theoretical basis for TPD.
出处
《西南农业学报》
CSCD
北大核心
2012年第6期2093-2097,共5页
Southwest China Journal of Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项(YW-FZX2008-12
2009-12
2010-9)
海南省重点科技项目(90107)
现代农业产业技术体系建设专项资金(CARS-34-02A)
关键词
橡胶树
死皮
双向凝胶电泳
蛋白
质谱
Rubber tree （Hevea brasiliensis Muell. Arg. ）
Tapping Panel Dryness （TPD）
2-DE
Protein
Mass spectrometry
