摘要
目的 了解聚吡咯 (Polypyrrole ,PP)材料与周围神经组织的生物相容性 ,为PP作神经桥接物进行缺损神经桥接再生试验提供实验依据。 方法 运用电化学氧化法合成导电玻璃、硅胶管上的PP膜。取新生大鼠的坐骨神经与PP材料联合培养 ,倒置显微镜与扫描电镜形态观察。结果 电化学氧化法合成的PP导电性能均较好 ;导电玻璃的PP膜表面光滑 ,微孔较小 ;硅胶管内壁膜除同导电玻璃外 ,还可成絮状表面 ,微孔交织成网。神经组织的雪旺细胞在平滑的PP表面生长迁移良好 ,而絮状表面相对差。 结论 体外联合培养结果提示 ,PP与大鼠周围神经组织有较好的生物相容性 。
Objective To obtain experimental data about polypyrrole (PP) as nerve bridge for applications in nerve regeneration on severed nerve and to investigate the biocompatibility of PP with peripheral nerve tissue. Methods The membranous PP on the indium tin oxide (ITO) conductive borosilicate glass or the silicone tube were synthesized electrochemically. The sciatic nerve from (1 3)—day SD rat with PP was cocultured. Results The conductance of PP synthesized electrochemically was better. The surface of PP on ITO was smooth with fewer microporosities. There were 2 kinds of surface characterization on the silicone tube, one of which was similar to PP on ITO, another like catkin with more microporosities. The schwann cell's migrating from the nerve tissue growed better on the smooth surface of PP than that on the catkin surface of PP. Conclusions Experimental study of coculture in vitro shows that there is good biocompatibility between PP and peripheral nerve tissues of rats, the cells of the nerve tissues are fitter to grow or migrate on PP with smooth surface.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2000年第7期411-413,共3页
Chinese Journal of Trauma
基金
国家杰出青年科学基金资助项目!(39425006)
江苏省教委青蓝工程资助项目
关键词
坐骨神经
细胞培养
生物相容性
聚吡咯
Sciatic nerve
Cell culture
Biocompatibility
Polypyrrole
Eletrochemical polymerization
