摘要
目的 用短串联重复位点检测方法 ,评价组织工程化肌腱临床应用修复喙锁韧带损伤的效果。方法 1999年 9月采用人胚肌腱细胞与材料体外复合培养 ,构建组织工程化肌腱 ,修复喙锁韧带损伤。术后 6个月 ,行内固定取出术时 ,钳取微量组织作组织学观察 ,并提取 DNA,检测 D3S175 4及 Cyar0 4基因座。结果 患者术后功能恢复良好 ,无局部及全身免疫排斥反应 ,D3S175 4及 Cyar0 4基因座电泳分型中含有非自体的等位基因 ,证实植入的组织工程化肌腱存活。
Objective To evaluate the result of clinical application in the repair of coracoclavicular ligament injury by tissue engineered tendon using the technique of short tandem repeat loci examination.Methods In september 1999, human embryonic tendon cells and artificial materials were co cultured in vitro to construct tissue engineered tendon, which repaired coracoclavicular ligament injury. After 6 months of operation, micro tissue were sampled during the operation of removal of internal fixation, and morphological characteristics were examined by HE staining, DNA of tissues were extracted to examine D3S1754 and Cyar04 gene loci. Results The shoulder function of the patient was recovered well after operation, and no local or systemic immunological rejection were occurred. The electrophoresis typing showed 13/14 at D3S1754 and 8/9 at Cyar04 in the tissue of tissue engineered tendon, while the autogenous ligament were 13/13 and 8/8 at D3S1754 and Cyar04 loci respectively, which suggested that the tissue engineered tendon was survived in vivo . Conclusion The examination of short tandem repeat loci is a better index to evaluate the survival situation of tissue engineered tissue after transplantation in clinical application.
出处
《中国修复重建外科杂志》
CAS
CSCD
2000年第4期237-240,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目!(39870202)
美国纽约中华医学基金会资助项目!(CMB98-682)
关键词
组织工程化肌腱
喙锁韧带
修复
短串联重复位点
Tissue engineered tendon
Coracoclavicular ligament Repair
Short tandem repeat loci
